摘要
目的 探讨黑素转铁蛋白 p97在人脑胶质瘤细胞铁摄取中的作用。 方法 应用含放射性柠檬酸铁(59Fe -Citrate)的培液孵育人脑胶质瘤细胞株GBM及BT32 5 ,以不同浓度PI -PLC及 pronase处理细胞 ,γ -闪烁计数仪测定cpm值 ,得到胶质瘤细胞的相对Fe摄取水平。结果 在一定浓度PI -PLC作用下 ,肿瘤细胞59Fe摄取水平显著降低 ,随着PI -PLC浓度的增加 ,加入 pronase和不加入pronase的细胞59Fe摄取量接近一致。
Objective To investigate the role of p97 (melanotransferrin) in iron uptake in human brain glioma cells.Methods Human brain glioma cell lines, GBM and BT325 were incubated in the medium containing 59 Fe-Citrate. The cells were treated with phosphatidylinositol-phospholipase C (PI-PLC) and pronase. The iron uptake of the cells was expressed as relative iron uptake level according to the cpm measured by the gamma scintillation counter.Results 59 Fe uptake of the cells was significantly declined with the certain concentration of PI-PCL. 59 Fe uptake of the cells treated with pronase tended to coincide with that of the cells treated without pronase in the increasing concentration of PI-PLC.Conclusion p97 expresses a high level and plays an important role in iron uptake in human brain glioma cells.
出处
《苏州医学院学报》
2000年第4期328-330,共3页
Acta Academiae Medicinae Suzhou
