摘要
目的 :本文通过检查配对母胎标本中的沙眼衣原体 (Ct) ,拟探讨Ct垂直传播的机制。方法 :应用细胞培养 ,聚合酶链反应 (PCR)检查配对母胎标本 (宫颈、羊水、新生儿睑结膜 )中的沙眼衣原体 (Ct)。结果 :10 2例剖宫产配对母胎标本中 ,宫颈、羊水、新生儿睑结膜均为阳性有 2 4例。宫颈阴性、羊水、新生儿睑结膜阳性共 6例。宫颈阳性、羊水、新生儿睑结膜阴性共4例。宫颈、羊水、新生儿睑结膜均为阴性有 6 8例。孕妇的Ct感染率 2 7.5 % (2 8/ 10 2 ) ,胎儿的Ct感染率为 2 9.4% (30 / 10 2 )。结论 :Ct宫颈感染与羊水细胞感染有非常显著的相关性 (P <0 .0 0 5 )。孕妇的Ct感染可导致胎儿感染 。
Objective: To study the modes and routes of the vertical Chlamydia trachomatis(Ct) transmission. Methods:Chlamydia trachomatis in the matched maternal-fetal samples(cervices, amniotic fluid and neonatal conjunctivas) was detected by cell culture and polymerase chain reaction(PCR). Results:Of the 102 matched maternal-fetal samples at caesarean birth ,24 matched samples of cervices, amniotic fluid and neonatal conjunctivas were positive for Ct by cell culture and PCR; 6 were negative for cervices but positive for amniotic fluid and neonatal conjunctivas; 4 were positive for cervices but negative for amniotic fluid and neonatal conjunctivas; 68 were negative for cervices, amniotic fluid ,and neonatal conjunctivas. The Ct transmission rates of pregnant women and newborns were 27.5%(28/102) and 29.5%(30/102), respectively. Conclusion:Cervical Chlamydia infection was highly related to the amniotic infection.The Ct infection in pregnant women may cause fetal infection and the pathways of intrauterine infection seem to be ascension from cervical access to the amniotic cavity.
出处
《重庆医科大学学报》
CAS
CSCD
2000年第2期142-143,161,共3页
Journal of Chongqing Medical University
关键词
沙眼衣原体
PCR
羊水
剖腹产
宫内感染
Chlamydia trachomatis
Polymerase chain reaction(PCR)
Amniotic fluid
