摘要
目的观察大鼠脑损伤后不同时间皮质神经细胞早期快反应基因ｃ－ｆｏｓ、ｃ－ｊｕｎ和ｃ－ｍｙｃ表达变化。方法Ｎｏｒｔｈｅｒｎ杂交、原位杂交和免疫组织化学方法检测皮质神经细胞中ｃ－ｆｏｓ、ｃ－ｊｕｎ和ｃ－ｍｙｃ的表达。结果脑损伤后１５ｍｉｎ，伤侧皮质神经细胞出现上述３种基因ｍＲＮＡ的表达，随时间的处长，表达逐渐增加，于伤后３ｈ达高峰并持续至伤后２４ｈ，伤后７２ｈ恢复至对照水平。ｃ－ｆｏｓ和ｃ－ｊｕｎ蛋白表达亦于伤后１５ｍｉｎ出现，ｃ－ｍｙｃ蛋白表达于伤后３０ｍｉｎ出现，并均于伤后３ｈ达高峰，持续至伤后６ｈ，伤后２４ｈ表达消失。结论脑损伤后上述３种基因表达特点与其它脑损害不同，可能与损伤机制和继发性神经细胞损害有关。
Objective To observe the expression of c-fos, c-jun and c-myc genes in cerebral cortical neurons following brain trauma in rats, Methods The expression of c-fos. c-jun and c-myc was detected by using Northern and in situ hybridization and immunocytochemistry Results The expression of the mRNA of c-fos, c-jun and c-myc genes was observed in neurons 15 min following the injury in the ipsilateral cerebral cortex, which increased gradually and reached their peak at h 6. lasting till h 24 and failed to be detected at h 72, c-fos and c-jun proteins expression were present 15 min after injury and c-myc protein after 30 min. peaking at h 3 and lasting till h6. and resumed normal level at h 24. Conclusions The expression characteristic of c-fos. c-jun and c-myc gene differs from that in other brain injures. which may be related to different injury mechanism and secondary neuronal damage,
出处
《第一军医大学学报》
CSCD
2000年第2期120-123,共4页
Journal of First Military Medical University
基金
军队九五杰出中青年人才基金!(96-M-83)
