摘要
目的观察miRNA-146a对血管平滑肌细胞凋亡的作用并研究其机制。方法原代培养大鼠血管平滑肌细胞，分成inhibitor组、control组和normal组，采用脂质体2000分别转染miRNA-146ainhibitors（50μM）、错义链（50μM）、PBS，realtimePCR测定转染后miRNA-146a水平，流式细胞仪检测转染后血管平滑肌细胞凋亡，western blot检测转染后Bax蛋白水平。结果转染48h后，inhibitor组血管平滑肌细胞的miRNA-146a水平明显低于normal和control组（P〈O．01），inhibitor组血管平滑肌细胞的凋亡显著高于normal、control组（P〈0．05），且Bax蛋白表达水平增加（P〈O．05）。结论miRNA-146a可以抑制血管平滑肌细胞的凋亡，其机制与抑制Bax表达相关。
Objective To observe the role of miRNA-146a in apoptosis of vascular smooth muscle cell and investigate its mechanisms. Methods Primary rat vascular smooth muscle cells were cultured,artificial synthesized miRNA-146 inhibitors（50μM） ,scrambles（50μM） and PBS were transfected into vascular smooth muscle cell by Lipofectamine 2000 individually. After transfection,flow cytometry was employed to measured apoptosis of vascular smooth muscle cell; Western Blot was employed to measure protein level of Bax in VSMC. Results After transfection 48h,compared with normal and control VSMCs, significantly lower miRNA-146a was observed in inhibitor VSMCs（P〈 0.01 ）. Meanwihle, inhibitor VSMCs showed higher apoptotic a bilityl P〈0.05 ） as well as higher protein level of Bax（ P〈 0. 05）. Conclusions MiRNA-146a is capable of suppressing apoptosis of VMSC,which is probably related with that Bax protein was inhibited.
出处
《中国误诊学杂志》
CAS
2012年第18期4860-4862,4865,共4页
Chinese Journal of Misdiagnostics
基金
深圳市科技计划项目（201102158）
