摘要
目的探讨RGC-32和E—cadherin蛋白在胰腺癌组织中的表达，分析其临床病理学意义以及两种蛋白表达之间的相关性。方法采用免疫组织化学sP法检测42例胰腺癌、12例慢性胰腺炎和8例正常胰腺组织中RGC-32和E—cadherin蛋白的表达。结果RGC．32主要表达于胰腺腺泡细胞胞质内；E—cadherin主要表达于正常胰腺和慢性胰腺炎组织胰腺腺泡细胞胞膜，而在胰腺癌中则可出现胞质表达和（或）表达减弱等异常表达。胰腺癌组织中RGC-32的阳性表达率以及E—cadherin异常表达率分别为78．6％（33／42）和54．8％（23／42），均显著高于正常胰腺组织的37．5％（3／8）和0以及慢性胰腺炎组织41．7％（5／12）和8．3％（1／12）（P值均〈0．05）。胰腺癌组织RGC一32表达与肿瘤淋巴结转移和TNM分期有关（P值分别为0．016、0．025），而与患者的年龄、性别及肿瘤分化程度无关（P值分别为0．831、1．000、0．629）；E—cadherin异常表达与胰腺癌分化程度、淋巴结转移和TNM分期有关（P值分别为0．024、0．004、0．004），而与患者年龄、性别无关（P值分别为0．970、1．000）。RGC-32的阳性表达率与E—cadherin异常表达率呈正相关（r=0．458，P〈0．01）。结论胰腺癌组织中RGC-32阳性表达率及E—cadherin异常表达率均显著升高，RGC-32可能通过调控上皮问质转化过程参与胰腺癌的侵袭和转移。
Objective To investigate the expressions of RGC-32 and E-cadherin in pancreatic cancer and analyze their clinicopathological significance and the correlation with each other. Methods SP immunohistochemistry was used to detect the expressions of RGC-32 and E-cadherin in 42 cases of pancreatic cancer tissues, 12 cases of chronic pancreatitis tissues and 8 cases of normal pancreatic tissues. Results The positive staining for RGC-32 was predominantly observed in the cytoplasm of pancreatic acinar cells. The positive staining for E-cadherin was mainly observed in the cytomembrane of normal pancreatic and chronic pancreatitis acinar cells, but aberrant expression （cytoplasm expression and （or） weaker expression） could be found in pancreatic cancer cells. The positive expression rate of RGC-32 and aberrant expression rate of E- cadherin were 78.6% （33/42） and 54.8% （23/42） , respectively, in pancreatic cancer tissues, which were significantly higher than those in normal pancreatic tissues [ 37.5% （3/8） and 0 ] and chronic pancreatitis [41.7% （5/12） and 8.3% （1/12） with statisticai significance, P 〈0.05 ]. The expression of RGC-32 in pancreatic cancer was associated with lymph node metastasis and TNM staging （P =0.016, 0.025, respectively）,but not with age, gender and differentiation degree （ P =0. 831 , 1. 000, 0. 629, respectively）. The aberrant expression of E-cadherin was associated with differentiation degree, lymph node metastasis and TNM staging （P = 0. 024,0. 004, 0. 004, respectively） , but not with age and gender （P = 0. 970,1. 000, respectively）. A significantly positive correlation was found between positive expression rate of RGC-32 and aberrant expression rate of E-cadherin （r =0. 458, P 〈0.01 ）. Conclusions Both positive expression rate of RGC-32 and aberrant expression rate of E-cadherin are up-regulated significantly in pancreatic cancer tissues and RGC-32 may he involved in the invasion and metastasis of pancreatic cancer by regulating epithelial mesen
出处
《中华胰腺病杂志》
CAS
2012年第3期173-176,共4页
Chinese Journal of Pancreatology
基金
教育部高等学校博士学科点专项科研基金（20110142110013）
关键词
胰腺肿瘤
免疫组织化学
RGC-32
上皮-钙黏素
上皮间质转化
Pancreatic neoplasmas
lmmunohistochemistry
Response gene to complement-32
Epithelial-cadherin
Epithelial-mesenchymal transition
