摘要
目的研究妊娠期母鼠缺氧对子代肾素-血管紧张素系统(RAS)及其调控的水盐代谢的"印迹"效应。方法妊娠母鼠随机分成缺氧组和对照组,缺氧组于妊娠第4～21 d放入缺氧舱(10.5%O2),对照组同期放入缺氧舱(21%O2)。在妊娠21 d(GD21)测量对照组和缺氧组胎鼠的脑质量、体质量及其血液电解质、血气、渗透压指标;检测两组成年子代(5个月)大鼠的血液电解质、血气及渗透压水平,记录皮下注射高渗盐水后两组成年子代大鼠的摄盐摄水量;Western blot检测两组成年子代前脑内血管紧张素受体(ATR)AT1R和AT2R蛋白表达的水平。结果妊娠期母鼠缺氧后,其胎鼠脑质量、体质量较对照组明显降低(P<0.05)。但成年子代鼠的脑质量、体质量与对照组比较,差异无统计学意义(P>0.05);缺氧组GD21胎鼠氧分压(PO2)、氧饱和度(SO2%)较对照组明显下降(P<0.05),成年子代缺氧组与对照组间的差异无统计学意义(P>0.05);胎鼠及成年子代血液电解质指标两组间的差异无统计学意义(P>0.05);缺氧组皮下注射高渗盐后的摄盐量明显增加(P>0.05),摄淡水量与对照组间的差异无统计学意义(P>0.05),且缺氧组子代脑组织中AT2R的蛋白表达明显降低(P<0.05),而AT1R蛋白表达与对照组间的差异无统计学意义(P>0.05),但AT1R/AT2R蛋白表达的比值明显升高(P<0.05)。结论孕期暴露于缺氧刺激可影响子代成年大鼠在皮下注射高渗盐水应激后的摄盐水平,且该变化可能与子代中枢血管紧张素受体的改变有关。
Objective To determine the effects of perinatal exposure to hypoxia on water-electrolyte metabolism related to rennin-angiotensin system （RAS） and ＂imprinting＂ effects in the offspring. Me- thods SD pregnant rats were individed into two groups randomly and were given different treatments. Fe- tal body weight, brain weight were measured at gestation 21 day （ GD21 ）. Blood gases, electrolytes and plasma osmotic pressure of both fetus and five-month old offsprings were measured. Intake of the 1.8% NaC1 and water was measured following subcutaneous injection hypertonic saline in the offsprings, and an- giotensin receptors in the brain were determined. Results Maternal hypoxia during gestation significantlydecreased GD21 fetal body weight, brain weight and plasma PO2and SO2% level, but there were no di- fferent in offsprings. And there was no different of blood Na ~/K ~ concentrations and plasma osmolality either in fetus or in adult offspring rats regardless of perinatal exposure to hypoxia. To the offsprings fo- llowing perinatal exposure to hypoxia, their salt appetite was significantly increased by subcutaneous injec- tion hypertonic saline. Furthermore, in the forebrain of the offsprings with perinatal exposure to hypoxia, expression of angiotensin AT2 R but AT~ R was reduced, and the ratio of AT~ R./AT2 R was significantly in- creased compared to control offspring. Conclusion The results showed that stimulated salt intake can be affected by exposure to hypoxia in fetal origins, and the changed behavior was associated with the remod- eled expression of AT1 and AT2 receptors in the forebrain of the offspring.
出处
《苏州大学学报（医学版）》
CAS
2012年第3期298-303,共6页
Suzhou University Journal of Medical Science
基金
国家自然科学基金重点资助项目(8130006)
关键词
妊娠期缺氧
子代
肾素-血管紧张素系统
水盐代谢
hypoxia during gestation
fetal and offspring
rennin-angiotensin system
water-electro-lyte metabolism
