摘要
目的:研究中药方剂组分配伍体外清除多环芳烃(PAHs)和自由基等致癌物质的作用。方法:按照均匀试验设计-药效试验-数学建模(模型验证)药效评价程序进行银杏叶提取物、甘草总黄酮、淫羊藿总黄酮、黄芪总苷和麦冬总皂苷5个中药组分不同配伍剂量体外清除PAHs和自由基的研究。结果:以清除1,1-二苯基-2-苦肼基(DPPH)、PAHs、羟自由基和超氧阴离子自由基的综合作用为指标,5味中药组分配伍后药效作用要强于单味药组分,其最佳配比剂量为甘草总黄酮0.8 g.L-1、银杏叶提取物3.144 g.L-1、淫羊藿总黄酮0.024 g.L-1、黄芪总苷0.036 g.L-1和麦冬总皂苷0.024 g.L-1。配伍最佳比例约为33∶131∶1∶1.5∶1。结论:中药组分配伍可进一步提高特定中药组分体外清除多环芳烃和自由基等致癌物质的作用,可使中药组分在减少此类物质对人群的危害方面发挥更好的作用。
Objective： To study the scavenging effect of active ingredient compatibility from Chinese herbal on carcinogens such as radicals and polycyclic aromatic hydrocarbons in vitro. Method： The active ingredient formula of Chinese herbal were composed of ginkgo biloba extract （GBE） , total flavonoids of glycyrrhiza （TFG） , total flavonoids of epimedium （TFE） , astragalosides （AST） and tatal saponins of ophiopogon （TSO）. Uniform design and comprehensive weight coefficient method was used to analyze the effect of the formula on cavenging polycyclic aromatic hydrocarbons and radicals in vitro. Result： According to the comprehensive action, the pharmacodynamic actions of five active ingredient formula was stronger than any single ingredient. The best effect was in the ratio of GBE 3. 144 g·L^-1 , TFG 0.8 g·L^-1X, TFE 0. 024 g·L^-1 , AST 0. 036 g·L^-1 and TSO 0. 036 g·L^-1. The optimum proportion was 33 ： 131 ： 1 ： 1.5 ： 1. Conclusion： Active ingredient compatibility of Chinese herbal could strengthen the scavenging effect of single active ingredients on carcinogens such as radicals and polycyclic aromatic hydrocarbons, which may play the role of reducing the damage of carcinogens on human being
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期183-187,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科学研究与技术开发计划课题(桂科攻11194005)
关键词
中药方剂组分配伍
自由基
多环芳烃
均匀设计
uniform design active ingredients assembling of Chinese herbal
radicals
polycyclic aromatic hydrocarbons
uniform design
