摘要
本试验旨在鉴定一株从肉鸡体内分离的广谱耐药菌,并从分子生物学角度对该株细菌的耐药机理进行探讨。根据《常见细菌系统鉴定手册》标准对该菌进行培养特性、生化试验鉴定,设计1对16SrDNA通用引物扩增其16SrDNA并测序鉴定;根据该菌的药物敏感性试验结果设计了12对引物扩增其基因组上耐药基因。细菌培养特性和生化试验结果显示该菌为一株肠球菌,PCR扩增出大小为1465bp的目的片段,药敏试验显示该株细菌耐受多种抗生素,PCR扩增其耐药基因发现存在ant(6)-iv、OXA-10、tetM、CTX-M-1、TEM基因。最终鉴定该株细菌为一株肠球菌,耐药基因的存在是其产生耐药性的原因之一。
This experiment was aimed at identifying a Broad-spectrum antibiotic resistant bacteria separated from chickens and discussing the mechanism of antibiotic resistance of the bacteria from the viewpoint of molecular biology. Identify the train- ing characteristics and biochemical experiments of the bacteria based on the standard of Common Bacteria System Identification Manual. A pair of 16S rDNA general primer was designed to amplify 16S rDNA and sequenced. 12 pairs of primer amplify re- sistance gene was designed based on the results of sensitive method of antibiotic test. The results of training characteristics and biochemical experiments of the bacteria showed that they were Enterococcus. A target set of 1465 bp was amplified by PCR. Sensitivity tests showed that the bacteria tolerate several antibiotics. The results of PCR showed that there exist ant（6）-iv, OXA-10, tetM, CTX-M-1, TEM gene, so it was Enterococcus. The existence of resistance gene was one of the reasons why the bacteria generate resistance characteristics.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第6期165-168,共4页
China Animal Husbandry & Veterinary Medicine
关键词
肠球菌
鉴定
耐药性
耐药基因
Enterococci
identification
drug-resistance
drug-resistance gene
