摘要
目的:用电导检测离子色谱法测定面制品中的溴酸盐(BrO3-)含量。方法:样品加入纯水超声提取,然后加沉淀剂沉淀蛋白、除去脂肪,上清液离心、过Ag/H柱除去氯离子,0.20μm滤膜过滤;选用DI-ONEX IonPac AS19分析柱分离,采用氢氧化钠溶液梯度淋洗,抑制型电导检测器检测。结果:溴酸盐在0.1μg/ml～5.0μg/ml浓度范围内具有良好的线性关系,r=0.9998;方法的检出限为0.40 mg/kg,相对标准偏差为3.72%～4.13%,方法回收率在90%～99%。结论:方法精密准确,快速灵敏,实用性强。
Objective：To determine bromate in flour and flour products by ion chromatography with suppressed conductivity detector.Methods：The bromate was first extracted from the samples by ultrasound with pure water,then precipitator was added to deposit protein and remove lipid,and the supernatant was centrifuged,then Ag/H column was employed to remove chloridion,then the solution was filtrated through 0.20 μm filter membrane,finally the suppressed conductivity detector was used for detection after separation with DI-ONEX IonPac AS19 and elution with sodium hydroxide solution.Results：The concentration of bromate showed a good linearity in the range of 0.1 μg/ml～5.0 μg/ml,r=0.9998,the detection limit was 0.40 mg/kg,RSD was 3.72%～4.13%,the recovery rate was 90%～99%.Conclusion：This method has advantages of high sensitivity and precision and precision,rapidity and convenience.
出处
《中国卫生检验杂志》
北大核心
2012年第6期1263-1264,1267,共3页
Chinese Journal of Health Laboratory Technology
关键词
溴酸盐
面制品
离子色谱法
Bromate
Flour products
Ion Chromatography
