摘要
目的 建立利用角蛋白检测外周血中循环癌细胞的方法 ,探讨角蛋白 19、2 0 (K19、K2 0 )在不同肿瘤细胞株和肿瘤组织中的表达。方法 采用定量逆转录多聚酶链反应 (RT PCR)方法检测11个肺癌、食管癌、膀胱癌、大肠癌肿瘤细胞株和 33例相对应的癌组织中特异性K19、K2 0mRNA的表达。结果 4种肿瘤细胞株及 33例相对应的癌组织均表达K19mRNA ,但表达量有明显差别。同种癌组织之间K19mRNA的表达量相差在 1.7～ 10倍之间 ,表达量最低者仅为阳性对照的 12 %以下。肺癌和食管癌细胞株和癌组织中均未见K2 0mRNA的表达。 6例膀胱癌组织中 ,仅有 1例呈阳性 ;5例肠癌组织中 ,4例呈阳性 ,表达量为阳性对照的 41%～ 77%。膀胱癌细胞株EJ1和肠癌细胞株SW480未见K2 0mRNA的表达。结论 K19、K2 0可作为检测外周血中循环癌细胞的生物标志物。但个别癌组织中K19、K2 0的表达低下 ,有可能导致检测出现假阴性结果。
Objective To study the expression of keratin 19,20 (K19, K20) in different tumor cell lines and tumor tissues and its clinical implication.Methods Quantitative reverse transcription polymerase chain reaction (RT PCR) was employed to examine the specific expression of K19 and K20 mRNA in eleven tumor cell lines and 33 corresponding tumor tissue specimens.Results The expression of K19 mRNA was detected in 4 kinds of tumor cell lines and all tumor tissues examined, but the magnitude of expression differed, with a difference ranging from 1.7 to 10 folds for the same type of cancer. In some patients, the level of expression was as low as 12% of the positive control. K20 mRNA expression was negative for lung and esophageal tumor cell lines and the corresponding carcinoma specimens. In one of 6 bladder cancer specimens and in 4 of 5 colorectal cancer tissues, K20 expression was positive, at a level of 41%～77% of the positive control. There was no expression of K20 in bladder tumor cell line EJ1 and colorectal tumor cell line SW480.Conclusion These results demonstrate that K19 and K20 may be used as a valuable marker for detecting circulating cancer cells, but the low level of expression in some cases of carcinoma would probably result in false negative results.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2000年第1期32-35,共4页
Chinese Journal of Oncology
基金
国家教委留学回国人员科研启动基金!( 1997 43 6)
关键词
角蛋白
聚合酶链反应
细胞株
循环癌细胞
肿瘤
Keratin
Polymerase chain reaction
Cell line
Circulating cancer cells
