摘要
目的探讨间变性淋巴瘤激酶(anaplastic lymphoma kinase,ALK)阳性的弥漫性大B细胞淋巴瘤(diffuse large B-cell lym-phoma,DLBCL)的临床病理及分子遗传学特点。方法对3例ALK(+)DLBCL进行光镜观察、免疫组化及荧光原位杂交检测,并复习相关文献。结果镜下观察淋巴结结构破坏,可见淋巴窦侵犯,肿瘤细胞大,呈免疫母细胞样,免疫组化示肿瘤细胞ALK、Bob-1、CD4、CD10、CD45、CD56、CD138、EMA、MUM1、Oct-2均(+),CD20、CD79a、PAX-5、CD3和CD30均(-),3例荧光原位杂交均检测到ALK基因易位。结论 ALK(+)DLBCL是一种DLBCL的少见独立亚型,具有特征性免疫表型、形态学及分子遗传学特点。
Purpose To study clinicopathological and genetic features of anaplastic lymphoma kinase （ALK）-positive diffuse large B- cell lymphoma （DLBCL）. Methods The clinicopathological and genetic features of 3 cases of ALK-positive LBCL diagnosed recently were analyzed with light microscopy, immunohistochemical staining and flurescence in-situ hybridization （FISH）, and the related liter- atures were reviewed. Results Histologic evaluation revealed a diffuse effacement of the nodal architecture by the infiltration of tumor cells, and sinusoidal infiltration was also seen. The neoplastic cells were large and exhibited the immunoblastic/plasmablastic morphol- ogy. By immunohistochemistry, all the cases showed a cytoplastic granular staining of ALK, Bob-l, CIM, CD10, CD45, CD56, CD138, EMA, MUM1, Oct-2, and kappa immunoglobuhn light chain, but negative for CD3, CD20, CD30, CD79a, PAX-5. FISH demonstrated the presence of ALK gene translocation in all of the 3 cases. Conclusion ALK （ ＋ ） DLBCL represents a distinct vari- ant of diffuse large B-cell lymphoma, and the tumor has a special histological features along with a distinct immunophenotypic profile and ALK gene rearrangement.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第6期653-656,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
淋巴瘤
免疫表型
荧光原位杂交
鉴别诊断
病理学
lymphoma
immomophenotyping
flurescenee in-situ hybridization
differential diagnosis
pathology
