摘要
文中介绍了2种较常用的外源总DNA直接导入植物的方法—花粉管通道法和离子束介导法,分别从原理与方法、发展与应用、机理与验证、问题与展望4个方面综述了这2种方法的创立与发展以及应用此方法取得的研究成果。
Two commonly used techniques of introducing exogenous total DNA into plant directly were introduced,which were pollen - tube path- way method and ion beam implantation method. The establishment, development and application achievements of the two techniques were intro- duced from their principles and methods, development and application, mechanism and verification, problems and prospect.
出处
《安徽农业科学》
CAS
2012年第17期9211-9213,9240,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(10505018)
农业部农业公益性行业科研专项经费项目(200803034)
河南郑州市科技局攻关项目(083SGYS33261-10)
关键词
外源总DNA
植物
花粉管通道
离子束介导
分子育种
Exogenous total DNA
Plant
Pollen - tube pathway
Ion beam implantation
Molecular breeding
