摘要
目的:福氏志贺菌的ipaB基因与大肠杆菌质粒pQE30融合,构建ipaB-pQE30重组质粒。方法:以福氏志贺菌2a2457T(Nalr)为模板,用PCR扩增ipaB目的基因片段,提取大肠杆菌pQE30质粒,经KpnI和SalI双酶切、DNA纯化后,用T4DNA连接酶将ipaB与pQE30进行连接。结果:ipaB-pQE30重组质粒接种在TOP-10中存活。结论:成功构建了ipaB-pQE30重组质粒。
Objective： Blending ipaB gene of Shigella flexneri and pQE30 particles of ba- cillus coil, structuring ipaB - pQE30 recom- bination particles. Methods： IpaB gene was amplified by PCR. The DNA products of ipaB were inserted into a prokaryotic expression vector pQE - 30, then translated into E. coli strain Topl0, restriction digestion and connecting. Results ： The ipaB - pQE30 recombination particles is living at TOP - 10 of bacillus coll. Conclusions：The results indicated that ipaB - pQE30 recombination particles was structured successfully.
基金
云南省教育厅重点资助立项课题(No:2009z0078)
