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志贺菌感染的多重PCR快速诊断技术的建立 被引量:3

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摘要 目的探讨多重PCR技术在志贺菌感染快速诊断中的应用。方法用多重PCR法对临床分离的68株志贺菌毒力基因shet1A、shet1B、ial和ipaH进行扩增检测。结果 68株志贺菌均在423bp(ipaH)处出现条带,在147bp(shet1B)、309bp(shet1A)、320bp(ial)处均出现了至少一个条带,与常规鉴定法的符合率达100%。结论成功建立了志贺菌感染的多重PCR快速诊断技术。
出处 《中国现代药物应用》 2012年第12期130-131,共2页 Chinese Journal of Modern Drug Application
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