摘要
目的探讨恶性肿瘤腹腔镜手术中电刀和超声刀电凝切割对肿瘤细胞的雾化作用。方法采用H22肝癌细胞制成小鼠实体肿瘤模型,对荷瘤小鼠分组用电刀、超声刀模拟恶性肿瘤腹腔镜手术,收集术中烟雾于PBS液,进行烟雾滤过液的涂片形态学观察、台盼蓝排斥试验和动物接种。结果烟雾滤过液涂片可见大量形态不规则的碳化微粒、细胞样大小碎片或圆形结构以及少量形态上相似细胞的物质结构,烟雾滤过液标本中未见台盼蓝拒染细胞,接种烟雾滤过液的小鼠未见肿瘤生长。结论电刀超声刀电凝切割实体肿瘤组织可引起肿瘤细胞雾化,这种细胞雾化作用在穿刺孔转移瘤发生机制中所起的作用不大。
[Objective] To investigate electrocautery aerosolization of malignant tumor cell. [Methods] Tumor models were produced by inoculation of H22 cancer cell in the right calf of mice. Laparoscopic surgery for malignant tumor was simulated by using electrome, UHS, respectively. Smoke particles were collected in PBS during the operation and then performed morphologic investigations of smears of suspensions, the trypan blue exclusion test and in vivo inoculation in syngeneic mice. [Results] A lot of carbonized particles, cell-sized rounded structures and a few morphologically similar cells were found in the smears of smoke suspensions. There were no trypan-blue-excluding cells in the smoke suspensions and no tumor growth in the mice inoculated with smoke suspensions. [Conclusions] It is possible that malignant cells may be aerosolized by application of cautery and cut tumor tissues. However, cellular aerosolization may play a small role in port site metastases.
出处
《中国内镜杂志》
CSCD
北大核心
2012年第5期454-457,共4页
China Journal of Endoscopy
基金
东莞市科技局基金(No.DG2010105150018)
关键词
恶性肿瘤
腹腔镜手术
穿刺孔转移瘤
细胞雾化作用
malignant tumor
laparoscopic surgery
port site metastases
cellular aerosolization
