摘要
采用蛋白免疫印迹杂交和免疫细胞荧光等方法首次研究了新型雌激素受体ERα36和窖蛋白-1(Caveolin-1)在大鼠皮肤组织中以及角质细胞系中的表达,探讨了二者的表达关系和分布特征.结果发现:(1)大鼠皮肤中有ERα36的表达,其表达的量与Caveolin-1的表达量呈负相关,且存在性别差异和部位差异;(2)表皮角质细胞中有ERα36的表达,主要分布在细胞膜上,且与Caveolin-1共定位.提示新型雌激素受体ERα36存在于皮肤细胞中,且可能参与Caveolin-1介导的雌激素信号转导,在雌激素治疗皮肤疾病过程中发挥一定作用.
In order to study on the expression and distribution of a novel estrogen receptor ERα36 in skin tissues of rats and keratinocytes,Western blot and immunoflourescence were performed.Result showed that（1）ERα36 is expressed in the skin of rats,and the relationship between amount of ERα36 and Caveolin-1 is negative correlation,the amount of difference not only in gender but also in tissue;（2） ERα36 is expressed in keratinocytes,and partly co-localized on the cell membrane with Caveolin-1.It suggested that ERα36 may participate in the estrogen signal pathway mediated by Caveolin-l and plays an important role in the process of estrogen treatment on skin.
出处
《辽宁师范大学学报（自然科学版）》
CAS
2012年第2期241-245,共5页
Journal of Liaoning Normal University:Natural Science Edition
基金
国家自然科学基金项目(30970353)
