摘要
目的:探索一种具有较高培养成功率的羊水细胞培养技术及其在产前诊断中的应用。方法:238例孕16～30周且有产前诊断指征的孕妇,在无菌条件下行羊膜腔穿刺术采集羊水进行细胞培养和染色体核型分析。实验过程对标本的采集、细胞接种收获和制片等环节进行了改进。结果:238例孕妇羊水成功培养231例,成功率97.1%,发现染色体异常核型8例,异常率占3.5%;其中染色体数目异常6例,嵌合体1例,平衡易位1例。结论:改进后的羊水细胞培养染色体核型分析技术是孕中期产前诊断胎儿染色体病的一种安全、有效、可靠的方法。
Objective：To explore an amniotic cell culture technology with higher harvests and verify its validity in prenatal diagnosis. Methods ： The amniotic fluid samples were obtained in 238 pregnant women with gestational weeks of 16 to 30 and indications for prenatal diagnosis by aseptic amniocentesis and cultured for determination of the chromosome karyo- type. In real performance, moderate improvement was done to the sample collection, cell inoculation and harvesting as well as aspects involved in the procedures. Results： Anmiotic cells were finally cultured in 231 of 238 cases（97.1% ）whose abnormalities were identified in 8 （3.5 % ）in which 6 were abnormal number of chromosomes, I mosaic and another 1, chromosomal balance translocation. Conclusion： The modified anmiotic cell culturing technique for kargotyping is effective in prenatal diagnosis of fetal chromosomal disease for its safety and reliability.
出处
《皖南医学院学报》
CAS
2012年第3期207-209,共3页
Journal of Wannan Medical College
基金
院人才引进基金项目(YJRC200904)
关键词
羊水细胞
培养
产前诊断
染色体核型
amniotie cell
culture
prenatal diagnosis
chromosome karyotyping
