摘要
依据生物矿化的基本原理,以蛋清蛋白为基质,透析袋组成隔室,在仿生条件下,诱导碳酸钙的合成。扫描电子显微镜和红外测试结果表明,半透膜和蛋白质虽然直接影响碳酸钙的形貌,但在半透膜和蛋清蛋白调控下生成的碳酸钙均为球霰石型晶体。实验结果表明,半透膜的选择性透过功能不仅限制了蛋白质的自由移动,同时也限制了蛋白质和Ca2+配合物的自由移动,从而为制备特殊形貌的碳酸钙固体提供了基础。
Based on the basic principles of biomineralization, calcium carbonate was induced by egg white protein (EWP) in the dialysis bag compartment under biomimetic conditions. The different morphology and polymorphy of calcium carbonate were characterized by SEM and FT - IR spectra. The results showed that calcium carbonates were all vaterite under different conditions except in the dis- tilled water. Dialysis bag limited not only the movement of protein, but also the movement of the coordination compound of Ca2+ - egg white protein, and provided a solid foundation to prepare a special morphology of calcium carbonate.
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第6期696-699,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金(21101054)
安徽省高等学校省级自然科学研究项目(KJ2011Z312
KJ2012B146)
安徽省高等学校优秀青年人才基金项目(2012SQRL167ZD)
合肥师范学院大学生科技创新基金资助(2011-2012)
关键词
仿生合成
碳酸钙
蛋清蛋白
透析袋
biomimetic synthesis
calcium carbonate
egg white protein(EWP)
dialysis bag