摘要
目的:揭示TEM1其与非小细胞肺癌侵袭和转移的可能关系,为靶向治疗提供理想的药物作用靶点。方法:实时荧光定量PCR方法检测56例非小细胞肺癌肿瘤组织及癌旁组织中TEM1 mRNA表达水平,分析其在不同组中的表达差异。结果:TEM1在56例非小细胞肺癌组织中都有表达。TEM1表达水平在肿瘤组织中比癌旁组织表达高,并且其表达水平与淋巴结转移及肿瘤分期密切相关(P<0.05),但与患者的病理类型,年龄及性别无关(P>0.05)。结论:TEM1表达水平与非小细胞肺癌分期密切相关,表明其可能是一个参与非小细胞肺癌侵袭及转移有价值的分子标记物。TEM1可能成为潜在的基因治疗靶点。
Objective： To investigate the role of TEM1 as a therapeutic target in human endothelial cells.Methods： The mRNA expression levels of TEM1 in tumor and corresponding paratumor tissue of NSCLC in 56 cases were detected by reverse quantitative transcription polymerase chain reaction（QRT-PCR）.And t tests were used to analyze statistical significances of their expression in different groups.Results： Analysis of 56 arrayed gradeⅠ-Ⅲ NSCLC demonstrated TEM1 expression in all tissues.mRNA levels of TEM1 were higher in tumor tissue than those in corresponding paratumor tissue.TEM1 expression was correlated with lymph node metastasis and clinical stage（P0.05）,but was independent of histological type,patient＇s age and gender（P0.05）.Conclusion： The expression level of TEM1 is correlated to clinical stage in NSCLC.It＇s probable a valuable molecular marker involved in NSCLC invasion and metastasis.TEM1 expression profile in NSCLC supports efforts to therapeutically target this protein,might be potential targets for gene therapy.
出处
《现代生物医学进展》
CAS
2012年第15期2904-2908,共5页
Progress in Modern Biomedicine
基金
Science and Technology Plan Projects of Qingdao(06-2-2-5-nsh-1)
the Development of Science and Technology Plan Projects of Shandong Province(2010GWZ20260)~~
