摘要
目的观察丙泊酚对LPS激活后人体中性粒细胞（PMN）释放炎性细胞因子的影响。方法 20例健康志愿者外周静脉采血,分离PMN后每例分为5份,分别加入等量生理盐水（C组,对照组）、LPS组（终浓度为100 ng/L）、LPS＋丙泊酚2μg/mL组（P1组）、LPS＋丙泊酚4μg/mL组（P2组）、LPS＋丙泊酚8μg/mL组（P3组）的5组。孵育12 h后,ELISA法检测培养液中TNF-α、IL-8含量,Western blotting法检测核内p65蛋白含量。结果实验剂量的丙泊酚均可不同程度抑制LPS激活后培养液中TNF-α、IL-8的增高（P〈0.05）;2~8μg/mL浓度的丙泊酚可以显著增加LPS导致的核内p65含量的增加（P〈0.05）;丙泊酚的这种效应具有剂量依赖性。结论 2~8μg/mL浓度的丙泊酚可通过抑制p65的激活,显著减少PMN释放炎性细胞因子。
Objective To study Propofol oninflammatory cytokines released by LPS-activate human polymorphonuclear in vitro and its mechanisms.Methods 20 cases peripheral blood were collected through healthy volunteers,after PMN was isolated,each case divided into five copies and added to normal saline（group C,control group）,LPS group（final concen tration was 100 ng/L）,LPS＋Propofol 2 μg/mL group（P1 group）,LPS＋Propofol 4 μg/mL group（P2 group）,LPS＋ Propofol 8 μg/mL group（P3 Group） respectively.After incubated for 12 hours,Tumor necrosis factor–α（TNF-α） and Interleukin-8（IL-8） content in culture supernatants was measured by Enzyme-linked immunosorbent assay（ELISA）;and intranucle arcontent of p65 protein was detected by Western blotting;PMN apoptosis was detected by flow cytometers.Results The designed dose of 2-8 μg/mL propofolreduced LPS induced inflammatory cytokines release through inhabit p65 protein acti vation（P 0.05） in a dose-dependent manner.Conclusion 2-8 μg/mL Propofol reduced LPS induced inflammatory cy tokines release in human polymorphonuclear through inhabit p65 protein activation.
出处
《中国医药导报》
CAS
2012年第17期95-96,共2页
China Medical Herald
基金
江苏省无锡市卫生局科研项目(项目编号:XM0805)
