摘要
目的:探讨光滑假丝酵母菌耐三唑类抗真菌药物临床株耐药基因表达情况。方法:34株复发性外阴阴道假丝酵母菌病的临床分离株,通过26S rDNA D1/D2区分子鉴定为光滑假丝酵母菌,应用法国生物梅里埃酵母菌药敏试剂盒ATBTMFUNGUS3,进行体外药物敏感性试验。实时荧光定量PCR方法检测34株菌株耐药基因CDR1、CDR2、SNQ2表达量。结果:34株光滑假丝酵母菌中,有2株为敏感株,2株对ITR剂量依赖敏感,30株为耐药株,且呈现交叉耐药现象。对氟康唑(FCA)、伊曲康唑(ITR)、伏立康唑(VRC)抗真菌药物的耐药率分别为26.32%、86.84%、34.21%。耐药株CDR1、SNQ2基因表达明显高于敏感株(P<0.0005),CDR2表达量无差别(P>0.05)。结论:光滑假丝酵母菌对三唑类药物MIC值高,其药物敏感性低与耐药基因CDR1、SNQ2的过度表达有关。
Objectives： To investigate the gene expressions of smooth candida mycoderma bacteria in resistantance to tria- zoles antifungal drugs. Method ： Thirty - four samples identified as smooth candida mycoderma bacteria through 26S rDNA D1 / D2 area, were administered with drug sensitivity test through French Merry biological medicine sensitive kit yeast ATBTM FUN- GUS3. Real - time fluorescence quota polymerase chain reaction （PCR） was used to detect the expression of resistance genes CDR1, CDR2, SNQ2. Results： Of 34 strains of smooth candida mycoderma, two were sensitive strains, two were dose -depend- ent on ITR, and 30 were drug - resistant strains presenting cross resistance phenomenon. The resistance rates to FCA, ITR, VRC were 26.32% , 86.84%, 34.21% respectively. The expression rate of resistance genes CDR1, SNQ2 was significantly higher than that of the sensitive strains （ P 〈 0. 0005 ） , but no difference was seen in CDR2 expression （ P 〉 0.05 ）. Conclusion ： The MIC value of candida giabrata to triazoles was high. Its low drug sensitivity is related to the over - expression of resistance genes CDR1. SNO2.
出处
《中国性科学》
2012年第6期3-6,12,共5页
Chinese Journal of Human Sexuality
基金
广东省人口与计生委科研立项项目(项目编号:2009308)
深圳市龙岗区科技计划医疗卫生立项项目(项目编号:YL-2009030)
