摘要
目的:探讨苦参素注射液与顺铂(DDP)联合对人肝癌SMMC-7721细胞端粒酶活性及wtp53、hTERTmRNA表达的影响。方法:采用MTT法测定细胞的增殖抑制率,应用金氏公式分析药物间的相互作用,TRAP-ELISA检测端粒酶活性,半定量RT-PCR法检测wtp53和hTERTmRNA表达。结果:苦参素注射液、DDP单独应用均能抑制SMMC-7721细胞增殖,两药联合具有单纯相加或协同作用,与DDP单药组相比,联合用药组的细胞抑制率明显增加(P<0.01),端粒酶活性显著下降(P<0.01),wtp53mRNA表达明显升高(P<0.01),hTERTmRNA表达明显减少(P<0.01)。结论:苦参素注射液和DDP联合应用具有单纯相加或协同抗肝癌SMMC-7721细胞增殖的作用,其机制可能通过上调wtp53和下调hTERT基因表达,从而有效抑制端粒酶活性。
Objective To investigate the effect of matrine injection combined with cisplatin on telomerase activity and wtp53, hTERT mRNA expression of human hepatocarcinoma cell line SMMC27721. Methods The inhibitory rate was evaluated by using MTT method. The interaction between matrine and cisplatin was analyzed by means of Jin＇s formulation. Telomerase activity was analyzed by TRAP-ELISA. The expression of wtp53 and hTERT mRNA were detected by semi-quantitive RT-PCR. Results Matrine injection and cisplatin used alone could inhibit the proliferation of SMMC27721 cells, and there was simple addition or synergistic effect after treated with combined agents. Compared with single drug cisplatin group, the inhibitory rate was increased significantly （P 〈 0.01 ）, telomerase activity was decreased markedly （P 〈 0.01 ）, the expression of wtp53 mRNA was increased significantly （P 〈 0.01 ） while hTERT mRNA was decreased markedly （P 〈 0.01 ）. Conclusion Matrine injection combined with cisplatin can obviously inhibit proliferation of SMMC27721 cells and display simple addition or synergistic effect, and its mechanism may be involved in inhibiting telomerase activity by up-regulating expressions of wtp53 and down-regulating expressions of hTERT gene.
出处
《实用医学杂志》
CAS
北大核心
2012年第11期1759-1761,共3页
The Journal of Practical Medicine
基金
广西自然科学基金资助项目(编号:0542119)
广西高校优秀人才资助项目(编号:RC20077029)
广西卫生厅重点课题(编号:重200887)
广西教育厅课题(编号:桂教科研200510197)
