摘要
旨在研究MSTN和p21基因在鸡、鹌鹑和杂交禽胚胎肌肉发育过程中的表达规律,比较这2个基因在杂交禽与亲本鸡和鹌鹑中的差异表达,探讨MSTN和p21基因与肌肉发育的关系。通过人工受精获得鸡(♂)与鹌鹑(♀)杂交禽蛋,与鸡蛋、鹌鹑蛋按照鸡标准孵化条件同批入孵,采集第7～17天活胚胸肌组织,应用实时荧光定量PCR技术检测MSTN和p21基因在3个物种中每一天mRNA的相对表达量。MSTN和p21基因在胚胎肌肉发育的第7～17天均有表达,这2个基因mRNA的表达在鸡胚中第9天到达第1次峰值,在鹌鹑中第7天到达第1次峰值,后都随胚胎的发育表达水平上升,并维持相对稳定的高水平表达。杂交禽的表达规律与鸡一致,也在第9天达到峰值,而p21mRNA表达极显著高于鸡和鹌鹑(P<0.01)。MSTN mRNA表达规律与成肌细胞退出细胞周期的时间规律一致;在胚胎肌肉发育过程中,MSTN基因特异性上调p21的表达。
The purpose of this study was to analyze MSTN and p21 mRNA expression profiles and differences among chicken, quail and hybrids of chicken-quail during embryonic myogenesis, and evaluate the potential relationships between their relative expression and myogenesis. The cross-bred eggs of quail （♀） were acquired by artificial insemination with chicken（♂）-semen, the eggs of chicken, quail and hybrid were hatched in one batch according to the standard condi- tion of chicken, the breast muscle tissues was collected from day 7 to 17. The expression of MSTN and p21 mRNA in different species every day was revealed by real-time quantitative PCR. The expression of MSTN and p21 mRNA was detected from day 7 to 17. Peak of MSTN and p21 expression was noticed at day 9 in chicken, and day 7 in quail, with the development of muscle, the expression level was rising, which was almost maintained at this level until day 17. And the peak expression was also noticed at day 9 in hybrid which was in accordance with chicken, howev- er, the expression of p21 mRNA in hybrid was significantly higher than that in chicken and quail （P〈0.01）. The expression profile of MSTN was in accordance with the profile of the withdraw- al of myoblasts from the cell cycle, and MSTN could up-regulated the expression of p21 specific- ally during embryonic myogenesis.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第6期997-1002,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
