摘要
背景:Faecalibacterium prausnitzi(FP)是人体肠道中常见的共生厌氧菌,与炎症性肠病的关系密切。目的:研究FP对人外周血单核细胞(PBMCs)白细胞介素(IL)-10、IL-12分泌以及CD25+ Foxp3+ Treg细胞分化的影响。方法:将FP、长双歧杆菌、大肠杆菌、不同浓度的FP上清或不同细菌培养基分别与人PBMCs体外共培养后,采用ELISA法检测细胞培养上清液中IL-10和IL-12浓度;流式细胞术检测CD25+ Foxp3+ Treg细胞比例。结果:与培养基相比,FP、长双歧杆菌均可刺激PBMCs分泌大量IL-10和IL-12,IL-10/IL-12比值明显增高,CD25+ Foxp3+ Treg细胞分化明显增加,且FP的效果优于长双歧杆菌。FP上清对IL-10/IL-12比值的作用呈浓度依赖性,稀释10倍的FP上清组IL-10/IL-12比值升高最为明显,且CD25+ Foxp3+ Treg细胞分化能力亦最为显著。结论:FP及其上清均具有抗炎作用和促进外周血CD25+ Foxp3+ Treg细胞分化的能力,提示FP是一种潜在的益生菌。
Background： Faecalibacterium prausnitzii （FP） is a commom anaerobic bacteria species colonized in human gut and there is a close relationship between FP and inflammatory bowel disease. Aims： To investigate the effect of FP on secretion of interleukin （IL）-10 and IL-12 and differentiation of CD25 ^＋ Foxp3 ^＋ Treg cells in peripheral blood mononuclear cells （PBMCs）. Methods： Human PBMCs were co-cultured in vitro with FP, Bifidobacterium Iongum （B. longum ）, Escherichia coli, different concentrations of FP supernatant and bacterial culture medium, respectively. Concentrations of IL-10 and IL-12 in culture supernatant were measured by ELISA. The proportion of CD25 ^＋ Foxp3 ^＋Treg cells was determined by flow cytometry. Results： Compared with culture medium, FP and B. longum showed a significantly higher induction of IL-10 and IL-12 and resulting in a higher IL-10/IL-12 ratio; proportion of CD25 ^＋ Foxp3 ^＋ Treg cells was significantly increased in FP and B. longum groups. The effect of FP was superior to that of B. longum. FP supernatant showed an effect of elevating IL-10/IL-12 ratio in a concentration-dependent manner. The IL-10/IL-12 ratio and proportion of CD25 ^＋ Foxp3 ^＋ Treg cells were the highest when FP supernatant was diluted by 10 times. Conclusions： Both FP and its supernatant display an anti-inflammatory capacity and the capability of inducing peripheral blood CD25 ^＋ Foxp3 ^＋ Treg cells differentiation, which indicates that FP may be a potential probiotics.
出处
《胃肠病学》
2012年第5期283-287,共5页
Chinese Journal of Gastroenterology
