摘要
目的检测乳腺癌扩增基因1（Mm）与上皮细胞钙黏蛋白（E—cadherin）在卵巢癌组织中的表达，并确定其表达与卵巢癌临床病理特征的关系。方法采用免疫组织化学EnVision二步法染色检测50例卵巢癌组织和13例正常卵巢组织中AIB1、E—cadherin、雌激素受体（ER）、孕激素受体（PR）与Ki。67等的表达。结果卵巢癌组织中AIB1阳性表达率[68％（34／50）]显著高于正常卵巢组织[8％（1／13）]（P〈0．01），E-cadherin表达下调率为60％（30／50）。AIB1阳性表达率在国际妇产科联盟（FIGO）分期Ⅲ＋Ⅳ期高于Ⅰ＋Ⅱ期（P=0．036），有淋巴结转移高于无淋巴结转移（P=0．027），Silverberg分期G，期高于G。＋G2期（P：0．003），浆液性腺癌高于非浆液性腺癌（P=0．049），ER阳性高于ER阴性（P=0．000），Ki-67阳性高于Ki．67阴性（P=0．009）；E—cadherin表达下调率在FIGO分期Ⅲ＋Ⅳ期高于I＋Ⅱ期（P=0．044），浆液性腺癌高于非浆液性腺癌（P=0．002），ER阳性高于ER阴性（P=0．021），Ki．67阳性高于Ki．67阴性（P=0．035）。AIB1与E-cadherin表达呈负相关（P=0．026）。结论卵巢癌组织中AIB1与E—cadherin表达情况及临床分期密切相关，可能是参与卵巢癌发展的重要分子。
Objective To examine the expressions of amplified in breast cancer 1 （AIB1） and epithelial cadherin （E-cadherin） in ovarian carcinoma （OC） tissues, and determine the correlation between the expression and clinical pathological features. Methods The expression of AIB1 ,E-cadherin,estrogen receptor （ER）, progesterone receptor （PR） and Ki-67 in tissues of 50 OCs and 13 normal ovarians tissues were detected by immunohistochemistry （IHC） EnVision two step process analysis. Results Positive expression of AIB 1 in OC tissues [ 68% （34/50）] was obviously higher than that in normal ovarian tissues [8% （1/13）] （P 〈0.01）. Down-regulation of E-cadherin expression was 60% （30/50）. The positive expression of AIB1 was significantly higher in stage Ⅲ and Ⅳ than in stage I and Ⅱ according to International Federation of Gynecology and Obstetrics （FIGO） stage （P = 0.036）, in lymph node metastasis group than in none lymph node metastasis group（P = 0.027）, in stage G3 than in stage G1 and G2 according to Silverberg stage （P = 0.003 ）, and in serous adenocarcinoma group than in non-serous adenocarcinoma group（P = 0.049） ;positive rates of ER and Ki-67 were higher than negative rates of ER （P = 0.000） and Ki-67 （P = 0.009） respectively. Down-regulation of E-cadherin expression was higher in FIGO stage m and Ⅳ than in stage I and Ⅱ （P = 0.044）, in serous adenocarcinoma group than in nonserous adenocarcinoma group （P = 0.022） ;positive rates of ER and Ki-67 were higher than negative rates of ER （P = 0.021 ） and Ki-67 （P = 0.035） respectively. The expression of AIB 1 was negatively correlated with E-cadherin expression （P = 0.026）. Conclusions The expressions of AIB1 and E-cadherin in OC tissues is closely related to clinical stage. Therefore, AIB 1 and E-cadherin may be important moleculars involved in the progression of OC.
出处
《中国医师进修杂志》
2012年第15期1-5,共5页
Chinese Journal of Postgraduates of Medicine
基金
广东省科技计划项目（20098030801317）
广东省医学科研基金项目（B2010228）
汕头大学医学院附属肿瘤医院青年基金资助项目[汕大医肿瘤医（2009）5号]
