摘要
实验观察了Ｄ－半乳糖（Ｄ－ｇａｌａｃｔｏｓｅ），超氧阴离了自由基Ｏ和Ｈ２Ｏ２对培养人胚肺二倍体成纤维细胞（Ｈｕｍａｎｆｅｔａｌ ｌｕｎｇ ｆｉｂｒｏｂｌａｓｔ，ＨＢＳ）的促老化作用。结果表明，Ｄ－十乳糖和Ｏ２均使细胞增殖速度减慢，分裂周期中Ｇ２－Ｍ期细胞比例减少、Ｇ－Ｇ１期细胞比例增加、ＤＮＡ含量下降，此外，细胞中ＳＯＤ含量降低，丙二醛（Ｍａｌｏｎｄｉｌｄｅｈｙｄｅ，ＭＤＡ）水平升高。Ｈ２Ｏ２上述作用不明显。提示，在Ｄ－半乳糖的代谢过程中可能产生了Ｏ２等活性氧自由基，Ｏ２本身和／或其与Ｈ２Ｏ２进一步反应生成的羟自由基（·ＯＨ）过量引起的组织细胞损伤及抗氧化酶活力下降、过氧化产物增多可能是Ｄ－半乳糖诱发细胞老化的主要原因。
The effects of O2 H2O2, and D-galactose on the cell senescence were investigated in human fetal lung fibroblast.The proliferative curves showed that the capability of cell proliferation decline treated byO2 [Xanthine (30 nmol/L) -Xanthine Oxidase (1 U/L) reaction system] and D-galactose (8 g/L), the proportion of the G-G, period cells increased but G,-M period cells decreased in comparison with those of control (D-glucose 8 g/L) and H2O2 (5 mmol/L) group. We have also observed the content of SOD decreasing but MDA increasing in both O, and Dgalactose group. The results found in D-galactose-treated cells were similar to those observed in O; and aged cell group, but had no changs in H,O, group, which is indicated that the reactive oxygen radicals (especially O,) were generated in the course of D-galactose metabolism.The oxidative stress might be responsible for the hastening the effect on cell senescence induced by D-galactose.
出处
《基础医学与临床》
CSCD
2000年第1期24-26,共3页
Basic and Clinical Medicine
