摘要
以三年生欧洲甜樱桃(Prunus avium L.)‘先锋’(砧木为马哈利)盆栽苗为试验材料,测定了不同光强对叶绿素荧光参数和D1蛋白含量的影响,以及NaHSO3和外源活性氧清除剂抗坏血酸(AsA)对叶片内H2O2积累的效应。结果表明,叶片在1 800μmol.m-2.s-1光强下处理6 h后,光系统Ⅱ最大光化学效率(Fv/Fm)、电子传递速率(ETR)、PSⅡ量子产量(ФPSⅡ)及非光化学淬灭系数(NPQ)下降,初始荧光(Fo)和光化学猝灭系数(qP)以及D1蛋白的相对含量变化不大。与对照(H2O)相比,叶片经5 mmol.L-1NaHSO3和4 mmol.L-1AsA处理后,Fv/Fm下降幅度减小。这些结果表明,强光下甜樱桃Fv/Fm的下降并非是光合机构遭受破坏所致而是光合功能下调的一种保护机制,低浓度的NaHSO3和AsA对保护光系统Ⅱ有一定作用。
Effects of different light intensity on chlorophyll fluorescence and the level of D1 protein in three-year-old sweet cherry（Prunus avium L.） variety Xianfeng grafted on P.mahaleb L.were investigated.The accumulation of H2O2 in leaves treated with NaHSO3 and exogenous ROS scavenger ascorbic acid（AsA） was also studied.The results showed that maximal photochemical efficiency of PSⅡ（Fv/Fm）,electron transport rate（ETR）,quantum yield of PSⅡ（ΦPSⅡ）,non-photochemical quenching（NPQ） declined,while initial fluorescence（Fo）,photochemical quenching（qP） and the relative content of D1 protein had no obvious changes in leaves under 1 800 μmol·m-2·s-1 radiation for 6 h.Compared with the control（H2O）,leaves treated with 5 mmol·L-1 NaHSO3 and 4 mmol·L-1 AsA had a less decline in Fv/Fm.These results indicated that the reduction in Fv/Fm was not a damage to the photosynthetic apparatus but a photoprotective strategy by down-regulating of photosynthetic function in sweet berry under high light,and low concentrations of NaHSO3 and AsA solution could protect the PS Ⅱ against photo-damage in cherry leaves.
出处
《浙江农业学报》
CSCD
北大核心
2012年第3期409-414,共6页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(30771497)
关键词
甜樱桃
叶绿素荧光
D1蛋白
强光
H2O2
sweet berry
chlorophyll fluorescence
D1 protein
high light
H2O2
