摘要
为了研究凝血酶对血管平滑肌细胞血小板源性生长因子基因表达的影响 ,探讨凝血酶刺激血管平滑肌细胞增殖的机制 ,通过培养SD大鼠胸主动脉血管平滑肌细胞 ,以3 H -TdR掺入率作为评价凝血酶促血管平滑肌细胞增殖的指标 ;用反转录聚合酶链反应检测凝血酶对血管平滑肌细胞血小板源性生长因子A链mRNA表达的影响 ;用Dotblot检测凝血酶对血管平滑肌细胞血小板源性生长因子B链mRNA表达的影响。结果表明 ,凝血酶促血管平滑肌细胞增殖呈浓度依赖性和时间依赖性 ,血管平滑肌细胞在基础状态下可检测到血小板源性生长因子A和B链mRNA表达 ,凝血酶刺激血管平滑肌细胞后 2h血小板源性生长因子A链mRNA表达开始增加 ,4～ 6h达高峰 ,持续 12h ,2 4～ 48h恢复正常。提示凝血酶对血管平滑肌细胞具有较强的促增殖作用 ,凝血酶促血管平滑肌细胞的增殖作用部分可能是通过诱导血管平滑肌细胞血小板源性生长因子A链mRNA的表达来实现的。
Aim [WT5”BZ]To study the influence of thrombin on gene expression of platelet derived growth factor(PDGF) in cultured vascular smooth muscle cells and probe into the mechanism of proliferation of vascular smooth cells induced by thrombin . [WT5”HZ]Methods [WT5”BZ]Take the incoporation of 3 H-thymidine as the target to evaluate vascular smooth muscle cells proliferation; thrombin-induced expression of PDGF-A mRNA in the vascular smooth muscle cells was detected by RT-PCR, while PDGF-B mRNA expression by Dot blot hybridization. [WT5”HZ]Results [WT5”BZ]Thrombin remarkedly stimulated proliferation of SD rat vascular smooth muscle cells in a dose-depedent and a time-dependent manner; PDGF-A and PDGF-B mRNA was detected in quiescent cultured vascular smooth muscle cells in serum-free medium for 48 hours,the expression of PDGF-A mRNA was increased in VSMC after exposure to thrombin for two hours, and reachd a peak levels after 4～6 houres exposure to thrombin. The rising level of PDGF -A mRNA continued for 12 hours,and felt to the base level 24～48 hours afterwards. [WT5”HZ] Conclusion [WT5”BZ] Thrombin remarkedly stimulated proliferation of SD rat vascular smooth muscle cells in a dose-depedent and a time-dependent manner, thrombin-induced vascular smooth muscle cells proliferation was partially mediated by expression of PDGF- A mRNA.
出处
《中国动脉硬化杂志》
CAS
CSCD
2000年第1期26-31,共6页
Chinese Journal of Arteriosclerosis
关键词
凝血酶
PDGF
VSMG
基因表达
血栓形成
Thrombin
Vascular
Muscle, Smooth
Platelet Derived Growth Factor
Gene Expression
Mice
