摘要
以胶原和肾上腺素混合液给小鼠 ,制造肺血栓模型 ,用放免法测定 6-酮 -前列腺素F1α ( 6-keto_PGF1α)和血栓素B2 (TXB2 )含量。结果表明 ,花椒油素 (Xanthoxylin ,XT ) 1 .0mg/kg,2 .0mg/kg,4 .0mg/kg和 8.0mg/kg使小鼠的存活率增加 2 8.6%~ 71 .2 % ;使 6_keto_PGF1α的产量提高 56.3 %~ 2 1 0 .8% ,使TXB2 的产量降低 2 1 .6%~ 56.8%。 6_keto_TGF1α与TXB2 的比值明显提高 ,对照组为 1 .1 5,药物组为2 .2 9,3 .1 3 ,5.53 ,8.2 6。体外实验证明 ,XT 0 .0 3 7μmol/L,0 .1 85μmol/L,0 .92 4 μmol/L,9.2 4 0 μmol/L显著地抑制血小板聚集和TXB2 产生 ,前者的抑制率为 2 2 .7%~ 55.3 % ,后者为 2 0 .5%~ 50 .7% ;但同时增加 6_keto_PGF1α的产量 ,提高率为 2 7.3 %~ 4 7.0 %。 6_keto_PGF1α与TXB2 比值 :对照组为 1 .2 3 ,药物组为2 .0 7,2 .4 4,3 .2 8,3 .85。
Model mice with pulmonary thromboembolism were established by administration of mixture of collagen and adrenaline.Radioimmunoassay was used to detect the serum level of 6_keto_prostaglandinF la (6_keto_PGF la ) and thromboxane B 2(TXB 2).Results showed that xanthoxylin being at the concentration of 1.0 , 2.0 , 4.0 , 8.0mg/kg ,the survival rate of mice was increased by 28.6%~71.2% ,the amount of 6_keto_PGF la by 56.3%~210.8%,and the amount of TXB 2 by 21.6%~56.8% .The ratio of 6_keto_PGF la and TXB 2 was enhanced markedly,being 1.15 in the control group, and 2.29 , 3.13 , 5.53 and 8.26 in the medication groups respectively.In vitro,xanthoxylin in the dosages of 0.037 , 0.185 , 0.924 , 9.240 μmol/L significantly inhibited the aggregation of platelet and the production of TXB 2,with the inhibition rate of 22.7%~55.3% and 20.5%~50.7% respectively.Meanwhile the amount of 6_keto_PGF la was increased by 27.3%~~47.0% and the ration of 6_keto_PGF la and TXB 2 was also increased,being 1.23 in the control group,and 2.07 , 2.44 , 3.28 and 3.85 in the medication groups respectively.
出处
《中药新药与临床药理》
CAS
CSCD
2000年第2期88-90,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
花椒油素
药理学
肺血栓
血栓素B2
药物作用
xanthoxylin/pharmacol.
@6-keto-PGF_(la)/drug eff.
@TXB_2/drug eff.
platelet aggregation/drug eff.