摘要
提出了高效液相色谱法同时测定水产品中呋喃唑酮、噁喹酸、萘啶酸及4种磺胺类药物等7种兽药残留量的方法。样品经乙腈提取,正己烷脱脂,用中性氧化铝柱和C18固相萃取柱进行分离及净化。以Symmetry C18色谱柱为分离柱,以不同体积比混合的乙腈与0.08mol.L-1乙酸溶液为流动相进行梯度洗脱,紫外检测器于波长265nm处检测。7种兽药的质量浓度与其峰面积均在0.05～1.0mg.L-1范围内呈线性关系,检出限(3S/N)为0.01～0.04mg.kg-1。方法的回收率在68.2%～97.3%之间,相对标准偏差(n=6)在1.6%～14%之间。
A method of HPLC for the determination of residual amounts of 7 veterinary drugs, including furazolidone, oxolinic acid, nalidixic acid and 4 sulfonamides in aquatic products was proposed. The sample was extracted with acetonitrile and defatted with n-hexane. The residue obtained was taken up with CH3CN-H2O （95-b 5） and purified by passing through neutral alumina column and C18 column. The eluate was separated on Symmetry C18 column by gradient elution with mixtures of acetonitrile and 0. 08 mol·L-1 acetic acid solution in different ratios, and determined with UV detector at the wavelength of 265 nm. Linear relationships between values of peak area ad mass concentration of the 7 veterinary drugs were obtained in the same range of 0. 05-10. 0 mg·L^-1 , with detection limits （3S/N） in the range of 0. 01--0. 04 mg·kg^-1. Values of recovery and RSD^s （n=6） of the method found were in the ranges of 68. 2%--97.3% and 1.6%--14% respectively.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2012年第5期543-546,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
江苏省科技基础设施建设计划(BM2008158)
江苏省水产三项工程(PJ2020-52)
