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低氧刺激对肾肿瘤细胞ADM基因表达的影响及意义 被引量：2

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摘要 目的探讨低氧刺激对肾上腺髓质素(ADM)在肾肿瘤细胞表达的影响。方法将生长良好的人肾肿瘤细胞786-o分为两组,实验组细胞低氧条件下培养3、6、12 h,对照组培养0 h,采用Westen-blot法检测四组ADM蛋白表达、RT-PCR法检测ADM mRNA表达。结果实验组3、6、12 h ADM蛋白和ADM mRNA均明显高于对照组,且呈时间依赖性。结论低氧刺激可诱导ADM在人肾肿瘤细胞中表达水平升高。
作者 王东彬 黎玮 杨书文 汪鑫 张艳平 乔娜 蔡文清
机构地区 河北医科大学第二医院
出处 《山东医药》 CAS 2012年第19期59-60,共2页 Shandong Medical Journal
关键词 低氧刺激 肾上腺髓质素 肾肿瘤
分类号 R737.11 [医药卫生—肿瘤]
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参考文献10

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共引文献9

同被引文献18

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引证文献2

二级引证文献10

山东医药
2012年 第19期

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