摘要
目的:不脱钙骨组织切片因组织有机及无机钙较多,其与普通石碏切片有较大差别。一种良好的防组织脱片剂是制片成功的关键因素之一,本研究的目的是寻找一种效果良好的防组织脱片剂以减少组织的损失,提高制片成功率。方法:选择目前组织病理制片中常用的三种防组织脱片剂对捞取切片的载玻片进行处理,防组织脱片剂分别为:3-氨丙基-3-乙氧基甲硅烷(APES)、铬明矾-明胶、多聚赖氨酸;另一种直接用已涂好防组织脱片剂(SUPERFROST PLUS)的进口玻片。组织切片经Goldners’三色法染色,比较分别使用上述四种防组织脱片剂的不脱钙骨组织切片组织保存的效果。结果:载玻片经3-氨丙基-3-乙氧基甲硅烷(APES);铬明矾-明胶;多聚赖氨酸;SUPERFROST PLUS等四种防组织脱片剂处理后,镜下观察:防组织脱片效果最好即组织保存最完整的是经铬明矾-明胶处理的玻片,完整贴片率为75%;多聚赖氨酸与SUPERFROST PLUS玻片两种基本相当,完整贴片率为30%;四种中效果最差的防组织脱片剂是3-氨丙基-3-乙氧基甲硅烷(APES),完整贴片率为15%。结论:通过对上述四种防组织脱片剂的应用比较,我们认为铬明矾-明胶作为不脱钙骨组织切片用防脱片剂是最理想的。
Objective： Undecalcification sections are quite different from paraffm sections due to the more organic and inorganic calcium.A good anti-slice escaping agentia is one of the key factors for success. The purpose of this study is to find an ideal anti-slice escaping agentia to reduce tissue damage and improve success rate. Methods： Three anti-slice escaping agentia including APES, chrome alum - gelatin and Poly-L-Lysine were used to pretreat slides, the other group was imported slides coated with SUPERFROST~ PLUS.The tissue sections were dyed with Goldners＇ staining, and the tissue preversation of the undecalcification sectins was studied. Results： The slides were processed respectively by APES, chrome alum- gelatin, Poly-L-Lysine and SUPERFROST~ PLUS, microscopy showed that the rate of complete adhesion in chrome alum - gelatin group was 75% which was the highest; the rates in Poly-L-Lysine group and SUPERFROST~ PLUS group were the same which was about 30%; and the rate in APES was the lowest which was 15%. Conclusion： Based on the comparison of four anti-slice escaping agentia, we conclud that chrome alum-gelatin is an ideal anti-slice escaping agentia for undecalcification sections.
出处
《中华老年口腔医学杂志》
2011年第6期329-331,共3页
Chinese Journal of Geriatric Dentistry
关键词
不脱钙切片
防脱片剂
APES
多聚赖氨酸
铬明矾-明胶
undecalcification section
anti-slice escaping agentia
APES
Poly-L-Lysine
chrome alum-gelatin
