摘要
研究了不同浓度的Hg2+胁迫不同时间(1～7 d)后,蛋白核小球藻(Chlorella pyrenoidosa)、斜生栅藻(Scenedesmus obliquus)和莱茵衣藻(Chlamydomonas reinhardtii)生长及叶绿素荧光特性的变化情况。测定的主要参数有光系统II(PSII)的最大光能转化效率(ΦM)、相对光合电子传递效率(rETR)、饱和光强(Ik)和细胞密度。试验结果表明,Hg2+胁迫下3种微藻的细胞密度和叶绿素荧光参数与Hg2+浓度、胁迫时间有一定的相关关系,细胞密度和叶绿素荧光各参数间有极显著的相关关系(P<0.01)。通过计算Hg2+对3种微藻不同胁迫时间的半抑制浓度(EC50),发现3种微藻对Hg2+的耐受性大小顺序为:斜生栅藻>莱茵衣藻>蛋白核小球藻。
The present study investigated the growth and chlorophyll fluorescence characteristics of 3 microalgal strains （ Chlorella pyrenoidosa, Scenedesmus obliquus and Chlamydomonas reinhardtii） under different Hg2 ＋ concen- trations and different durations （1 -7 d）. The main parameters including the maximal photochemical efficiency of PSII（ФM）, the relative electron transport rate （rETR）, the saturation intensity （Ik）and cell density were deter- mined. The results showed that the chlorophyll fluorescence and cell density of 3 microalgal strains all exhibit some correlations to Hg2＋ concentration and exposure duration. While cell density has very significant positive correla- tions （P 〈0.01） to fluorescence parameters. Comparison of the EC50 values of different exposure durations among the 3 algal species indicated the ability of their tolerance to Hg2＋ was S. obliquus 〉 C. reinhardtii 〉 C. pyrenoid- osa.
出处
《水生态学杂志》
北大核心
2012年第2期96-99,共4页
Journal of Hydroecology
基金
水利部948项目"藻类在线水体生态毒性监测系统"(合同号:200902)
环境保护部水体污染控制与治理科技重大专项(2009ZX07528-003)
关键词
微藻
Hg2+
叶绿素荧光
细胞密度
microalgae
Hg2 ＋
chlorophyll fluorescence
cell density
