摘要
为建立一种能快速检测肠炎沙门菌(SE)的方法,本研究根据基因库中SE种特异性基因(sdfⅠ)的保守序列,设计一套特异性环介导等温扩增(LAMP)引物,建立了SE的LAMP可视化检测方法。该方法的敏感性可达100fg DNA,高于常规PCR方法100倍;全部反应可在1h内完成;可通过肉眼观察颜色直接判定结果;对其他常见病原体的检测结果均为阴性。结果表明建立的LAMP方法简便、快速、灵敏、特异,可用于SE感染的快速检测。
A loop-mediated isothermal amplification（LAMP） assay was developed for detection of Salmonella enteritidis（SE）.According to the sequences specific to Salmonella difference fragment（SdfⅠ） of SE species in GenBank,six pairs of primers were designed,and the reaction conditions were optimized.The results showed that the detection limit of this LAMP method was 100 fg DNA,which was higher than that of routine PCR.The amplification could be finished within 1h,and the presence of SE could be detected by naked eyes.There was no cross-reactivity with other pathogens.These results suggest that this LAMP assay is a simple and specific method for rapid detection of SE in clinical samples.
出处
《动物医学进展》
CSCD
北大核心
2012年第5期27-30,共4页
Progress In Veterinary Medicine
基金
桂渔牧科09254-18
广西特聘专家专项资助项目(桂科攻1123007-4)
