摘要
目的 以曼陀罗种子为材料探讨太空环境对药用植物的影响。方法 利用返回式卫星搭载曼陀罗的种子 ,返回地面后播于实验田中 ,应用随机扩增多态性 DNA(RAPD)分子标记技术分析不同组曼陀罗基因组的变异情况。结果 从 6 5个供试引物中筛选出 15个能够产生可重复多态性扩增产物的引物。与地面对照组相比 ,失重组共产生 39条多态性片段 ,基因组的多态性频率为 2 3.1% ;击中组共产生 45条多态性片段 ,基因组的多态性频率为 2 4.4%。多态性片段大小在 2 0 0～ 190 0 bp之间。结论 微重力引起曼陀罗一定程度的基因组变异 ,微重力和高能离子辐射的复合效应大于微重力本身对植物的影响。
Objective To substantiate the effect of space environment on medicinal plants,the seeds of Datura innoxia were set up in retrievable satellites. Methods After returning to earth,the DNA variation of different groups was detected by random amplified polymorphic DNA (RAPD) markers. Results From a pre screening of 65 primers with 20 oligonucleotide lengths,15 primers that produced distinct profiles for each DNA sample were selected.In contrast with the earth controlled group,39 polymorphic bands were produced in weightless group,and its degree of gene polymorphism was 23 1%;45 polymophic bands were produced in hit group,and its degree of gene polymorphism was 24 4%.The polymorphic bands ranged approximately from 200 to 1990 bp. Conclusions These results indicate that weightlessness induce DNA variation to some extents in Datura innoxia, while the compound effect of weihtlessness and high energy heavy ions is more notable than the effect of single weightlessness.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2000年第1期44-47,共4页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金! ( 3 9670 0 82 )
关键词
曼陀罗
太空环境
遗传变异
RAPD
Datura innoxia
space environment
genetic variation
random amplified palymorphic DNA
