摘要
[目的]采用常用的分离培养肾细胞方法比较分离培养肾细胞,并提出优化方案。[方法]A法:组织块培养法;B法:1%胶原酶消化分离法;C法:5%胰蛋白酶消化法;D法:0.5%胰蛋白酶-0.02%EDTA连续分离法。[结果]A法初期细胞生长不好,1周后生长良好;B法、C法细胞贴壁状况较好,但细胞生长不均一,呈丘陵状生长,细胞产量较少;采用D法消化肾组织块,能够快速获得大量细胞,并且细胞生长整齐、均一,能够满足后续实验的需要。[结论]使用D法分离培养肾细胞优于其他3种方法,试验效果最佳。
[ Objective ] To compare the common methods to separate and culture the renal cells, and propose the optimized scheme. [ Method ] A ： Renal ceils were separated by tissue culture. B ： Renal cells were separated by 1% collagenase. C ： Renal cells were separated by 5 % trypsinase digestive juice. D： Renal cells were separated by successive 0.5% trypsinase- 0.02% EDTA digestive juice. [ Result] The renal cells grew poor at first by method A, however they grew well after one week. The attached cells from B and C were in good state, but they grew unevenly and the quantity was small; the method D digested the renal tissue, and could obtain a large quantity of cells, and the cells grew evenly, and could meet the needs of follow-up experiments. [ Conclusion ] Method D is better than other three methods to separate and culture the renal cells.
出处
《安徽农业科学》
CAS
2012年第15期8536-8538,共3页
Journal of Anhui Agricultural Sciences
基金
西南大学青年基金资助(2010RCQ001)
西南大学荣昌校区动物医学系教学改革项目
西南大学基本科研业务费专项资金(XDJK2012C097)
关键词
肾细胞
分离培养
生长
Renal cells
Separating and culturing
Growth
