摘要
[目的]通过测定2种菌株的抗氧化酶活性来研究其抗盐机理。[方法]以外生菌根真菌Paxillus involutus的2种菌株MAJ和NAU作为材料,研究在不同NaCl浓度(0、100、200、500 mmol/L)处理条件下抗氧化酶活性的变化。[结果]100 mmol/L NaCl可以促进菌株MAJ和NAU的增长及其生物量的增加;短期盐胁迫(1 d)诱导了菌株MAJ中SOD、CAT和POD活性的升高,但随着盐处理时间的延长,这些酶活性在100和200 mmol/L盐胁迫下均有所降低;而菌株NAU在长期盐胁迫(9 d)下能更好地维持各抗氧化酶活性,尤其是APX、GPX和POD。[结论]盐处理的菌株抗氧化酶活性明显高于对照菌株。
[ Objective ] Salt resistance mechanism of two strains of antioxidant enzymes was studied through the determination of their activity. [ Method ] The effects of different concentration of NaCl on antioxidative enzymes activity （SOD, CAT, APX, POD and GPX） of Paxillus involutus （strains MAJ and NAU） were investigated. [ Result] The growth and biomass of the two strains MAJ and NAU were both improved under 100 mmol/L NaCl treatment. Activities of anti-oxidative enzymes were increased in strain MAJ after a short-term salinity （ 1 d）. However, the activity of antl-oxidative enzymes declined with the increase of NaCl concentrations and the duration of salt treatment. Under a long-term salinity （9 d）, strain NAU remained the activities of anti-oxidative enzymes, especially APX, GPX and POD. [ Conclusion ] Anti-oxidantive enzymes activity of salt treatment were higher than those of control evidently.
出处
《安徽农业科学》
CAS
2012年第15期8401-8403,8405,共4页
Journal of Anhui Agricultural Sciences
基金
北京市优秀博士学位论文指导教师专项基金(YB20081002201)
教育部重点项目(209084)
国家自然科学基金项目(30872005)
北京市自然科学基金项目(6112017)
中央高校基本科研业务费专项资金(JC2011-2)
