摘要
本文利用离子液体(IL)和普鲁士蓝(PB)纳米方块的协同作用测定鸟嘌呤。首先制备了IL-PB修饰电极,用循环伏安法对修饰电极进行了表征。为了使PB自身的信号达到最大,优化了各种制备条件,如IL和PB的比例,KCl溶液和HCl的浓度等。使用制备的修饰电极催化鸟嘌呤,优化了鸟嘌呤的测定条件如B-R缓冲溶液pH值;疏水性离子液体和亲水性离子液体对鸟嘌呤的影响,结果表明疏水性离子液体催化效果更好。该法在最优化条件下检测鸟嘌呤,在4.0×10-7～1.4×10-6 mol/L范围内与氧化峰电流呈现良好的线性关系,检出限为6.0×10-8 mol/L。
In this paper,ionic liquids（IL）and prussian blue（PB）nanocubes were integrated to detect guanine.IL and PB were in turn cast on the glassy carbon electrode in a certain ratio to prepare the sensing platform.Cyclic voltammetry was adopted to characterize the modified electrode.In order to achieve maximum signal of PB,the optimized experimental conditions were pursued,such as the amount of IL,the concentration of KCl and so on.The experimental conditions were optimized in detail for electrochemical determination of guanine.The optimal experimental conditions were list as follow∶ volume ratio of IL and PB was 9∶7;KCl concentration was 0.1mol/L;scan rate was 100mV/s,pH value of B-R buffer solution was selected as 5.0.Guanine presented better electrochemical behavior at hydrophobic ionic liquids modified electrode compared with hydrophilic ionic liquid.Under the optimal experimental conditions,a direct electrochemical determination of guanine was achieved.The detection limit（S/N=3）for guanine was 6.0×10-8 mol/L.
出处
《分析科学学报》
CAS
CSCD
北大核心
2012年第3期308-312,共5页
Journal of Analytical Science
基金
山东省自然科学基金(No.2009ZRB019WB)
