摘要
【目的】 了解砷对体内细胞DNA的损伤作用及其机制。【方法】 在小鼠饮水中加入As2 O3,喂养 6个月 ,采用单细胞凝胶电泳技术对骨髓细胞DNA断裂进行了分析。【结果】 高剂量组 ( 12 5mg/L)小鼠骨髓细胞DNA的迁移度和迁移率显著高于阴性对照组 ,P <0 0 5 ;中、低剂量组 ( 2 5、0 5mg/L)小鼠骨髓细胞DNA的迁移度和迁移率与阴性对照组无显著差异。各剂量组另选 6只小鼠 ,以不引起明显DNA断裂剂量 ( 2 0mg/kg)的环磷酰胺进行腹腔注射 ,骨髓细胞DNA的迁移度和迁移率显著高于接受 2 0mg/kg环磷酰胺或As3O3单独作用的小鼠 (P <0 0 5 )。用蛋白酶K处理裂解后的细胞高剂量组的迁移度与迁移率显著高于未处理的细胞 (P <0 0 5 )。【结论】 高剂量的砷在小鼠体内对骨髓细胞DNA有损伤作用 ,并可诱发DNA 蛋白质交联 。
Objective To study the damage effect and its mechanism of Arsenic on DNA of cells in vivo. Methods \ The DNA break in bone marrow cells from the mice which have drunk the water containing As\-2O\-3 for 6 months were analyzed with Single cell gel electrophoresis. Results \ The length of DNA migration and the rate of DNA migration of the bone marrow cells increased remarkably at high dose(12\^5 mg/L)group( P <0\^05) and there were no significant difference between medium,low dos(2\^5,0\^5 mg/L) group and control group.Cyclophosphamide 20 mg/kg which do not induce DNA break was intraperitoneally injected to 6 mice each group,the length of DNA migration and the rate of DNA migration of the bone marrow cells were significantly higher than those of both control group which were injected cyclophosphamide only and same As\-2O 3 dose group which were not injected cyclophosphamide.The lysed cells were also treated with proteinase K, the values were higher than those which were not treated with proteinase K( P <0\^05). Conclusion \ High dose arsenic has damage effects on marrow cell's DNA of mice in vivo and induce DNA\|protein crosslink at same time.Lower dose arsenic can affect the sensitivity of DNA to other carcinogen.
出处
《卫生毒理学杂志》
CSCD
2000年第1期29-31,共3页
Journal of Health Toxicology
关键词
砷
骨髓细胞
DNA损伤
单细胞凝胶电泳
致癌物
Arsenic
DNA damage
Single cell gelelectrophoresis
DNA\|protein crosslink
