摘要
目的 :探讨P糖蛋白在骨肉瘤组织中的表达和其临床意义 ,以及与PCNA、P53 、nm -2 3蛋白表达的相关性。方法 :应用免疫组化方法 (S -P法 )测定P糖蛋白在 3 3例骨肉瘤组织中的表达。男 2 4例 ,女 9例 ,年龄平均 2 0 .6岁。ⅡA5例 ,ⅡB2 3例 ,ⅢB5例。同期采用相同方法测定PCNA、P53 、nm -2 3的表达。结果 :19例获得平均 1年 7个月随访 ,复发 3例 ,肺转移 11例 (其中 6例死亡 )。P糖蛋白阳性表达率为 45 .5 % ,与性别、年龄、部位及外科分期无关 ,与预后有关 ;与PCNA、P53 、nm -2 3蛋白表达无相关性。结论 :P糖蛋白的表达是骨肉瘤产生多药耐药的重要因素 ,并影响患者的预后 ,癌基因在骨肉瘤中的表达无相关性。
Objective: To investigate the expression of P glycoprotein in osteosarcoma and it's clinical significance,and the correlation with the expressions of PCNA、P 53 、nm-23 gene.Methods:the P glycoprotein expressions of 33 osteosarcomas were determined by immuno histochemistry(S-P) method.There were 24 cases in males.9 in females and with the mean age of 20.6 years old,5 in Ⅱ A,23 inⅡ B and 5 in Ⅲ B.the expressions of PCNA、P 53 、nm-23 were detected with the same method.Results:19 cases were followed-up with the average time of 1 year and 7 months,there were 3 cases in recurrent and 11 in lung metastasis (6 death among them).The positive expression rate of p glycoproteim was 45.5%,and no correlation with clinicalfactors,and related with the prognosis,no relationship with the expressions of PCNA、P 53 、nm-23 gene.Conclusion:The expression of p glycoprotion was an important factor of existing multidrug resistance in osteosarcoma,and influencing the prognosis,no relationship existed between the expressions of oncogene in osteosarcoma.
出处
《中国矫形外科杂志》
CAS
CSCD
2000年第3期224-226,共3页
Orthopedic Journal of China
关键词
骨肉瘤
P糖蛋白
免疫组化
Osteosarcoma
P glycoprotion
Imunohistochemistry
