摘要
测定海南栽培长裙竹荪中总糖的含量。以葡萄糖为标准品,5%苯酚溶液和浓硫酸为显色剂,采用分光光度法在490nm波长处测定样品的吸光度。葡萄糖标准品在(0.01～0.08mg/mL)范围内线性关系良好,其回归方程Y=-0.005+10.38333X,R2=0.9969。海南栽培长裙竹荪中总糖的含量为8.16%。分光光度法适合长裙竹荪中总糖的含量测定,该方法操作简便,结果相对稳定,重现性好。
To determine total saccharide in cultivated Dictyophora indusia Fisscher of Hainan.Under the experimental condition including glucose as the reference,5% phenol-sulphuric acid as color developer.The content of total saccharide in cultivated Dictyophora indusia Fisscher of Hainan was determined by spectrophotometric method at 490 nm.The calibration curve was linear（R2=0.9960） in the range of 0.01～0.08 mg/mL and the curve was Y=-0.005＋10.38333X.The content of total saccharide in cultivated Dictyophora indusia Fisscher of Hainan was 8.16%.The spectrophotometric method was suitable for the determination of total saccharide in cultivated Dictyophora indusia Fisscher of Hainan.This method is convenient,stable and repeatable.
出处
《食品科技》
CAS
北大核心
2012年第5期270-271,276,共3页
Food Science and Technology
基金
海南省教育厅高等学校科学研究资助项目(Hjkj2010-26)
关键词
长裙竹荪
总糖含量
分光光度法
Dictyophora indusia Fisscher
total saccharide
spectrophotometric method
