摘要
采用驯化的方法从活性污泥中分离到一株苯酚降解菌XTT-3,经16SrDNA鉴定为Sphingobiumsp.。对该菌株进行碳饥饿处理,发现其降解苯酚的能力受到抑制。以只含苯酚的M9培养基为参照,添加0.2g/L酵母膏作为共代谢基质,对XTT-3菌株降解苯酚有较明显的促进作用,36h后苯酚降解率为68%。在含0.2g/L酵母膏的M9培养基中,同时添加20mg/L邻苯二酚,XTT-3降解苯酚作用显著增加,24h后苯酚降解率达75%,苯酚降解速度达0.261mg/min。
A phenol-degrading bacterial strain XTT-3 was isolated from active sludge using acclimatization method.According to biochemical characteristics and 16S rDNA gene sequence analysis,the strain was identified as Sphingobium sp..The ability of phenol biodegradation was almost inhibited after the carbon starvation treatment of XTT-3.Compared to the control group（with phenol as the only metabolic substrate）,adding 0.2 g/L of yeast as co-metabolic substrate could enhance the phenol biodegradation,the phenol removal rate increased to 68% after 36 h of degradation.Re-supplementation of 20 mg/L catechol could further accelerate the biodegradation of phenol,75% of phenol was removal after 24 h of degradation,given the biodegradation rate of 0.261 mg/min.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2012年第5期39-42,共4页
Environmental Pollution & Control
基金
上海市教委青年基金资助项目(No.03DQ25)
上海师范大学科技基金资助项目(No.DKL714)
