摘要
目的:研究补中益气汤对甲状腺功能减退(甲减)大鼠心肌细胞凋亡及Fas,FasL和Caspase-3蛋白表达的影响。方法:采用Kolaja法制备大鼠甲状腺功能减退模型。健康Wistar大鼠120只随机分成正常对照组、模型组、补中益气汤组(18.9 g·kg-1)、左旋甲状腺素钠L-T4组(9.45μg·kg-1),各组灌胃给药56 d。光镜和电镜下观察心肌形态学和超微结构变化,TUNEL法检测心肌凋亡细胞,免疫组化检测凋亡相关基因蛋白Fas,FasL,Caspase-3的表达,ELISA法测定血清促甲状腺激素(TSH),放射免疫分析法测定血清总三碘甲状腺原氨酸(TT3)及总甲状腺素(TT4)。结果:与正常组相比,模型组心肌细胞凋亡指数及Fas,FasL,Caspase-3的表达均达高峰,心肌细胞结构损伤严重,经治疗后补中益气汤组(12.10±0.73)和L-T4组(29.26±2.10)的心肌细胞凋亡指数降低,Fas,FasL及Caspase-3的阳性表达均下降,且补中益气汤组的疗效优于L-T4组(P<0.05)。结论:甲减引起心肌细胞凋亡增多,Fas,FasL,Caspase-3表达升高,而补中益气汤可明显降低心肌细胞凋亡指数,下调Fas,FasL及Caspase-3的表达,说明补中益气汤对甲减的心肌损害有干预保护作用。
Objective ： To study the effect of Buzhong Yiqi Tang （tonifying middle-Jiao and Qi treatment） on myocardial apoptosis and expression of proteins Fas, FasL and Caspase-3 in hypothyroid rats. Method： The rat of hypothyroidism models were prepared by Kolaja method. Healthy wistar rats were randomly divided into normal control group, model group, Buzhong Yiqi Tang group, Letrox （L-T4） group. The morphological and ultrastructural changes were observed under optical microscope and electronic microscope respectively. Apoptotic ceils were measured by TdT-mediated dUTP nick end labeling （TUNEL） method. The expression of apoptosis-related proteins Fas, FasL and Caspase-3 were detected with immunohistoehemical methods. TSH was measured by ELISA, Totel 3 Triodothy ronine （ TT3 ） and Totel 4 Triodothy ronine （TT4） were measured by RIA; detect the iodine in urine. Result： Compared with control group, the apoptosis index and the expression of Fas, FasL and Caspase-3 reached the highest level in the model group, myocardial cell injured severely. After treatment the apoptosis index of Buzhong Yiqi Tang group （ 12. 10 ± 0. 73） and L-T4 group （29.26± 2.10） decreased, the expression of Fas, FasL and Caspase-3 also reduced, and the effectiveness of Buzhong Yiqi Tang group was better （P 〈 0. 05 ）. Conclusion： Hypothyroidism causes increase of myocardial cell apoptosis, heightened expression of Fas, FasL and Caspase-3, while Buzhong Yiqi Tang might decrease the myocardial apoptosis and lower the expression of protein Fas, FasL and Caspase-3 significantly, it indicated that Buzhong Yiqi Tang has protective action on myocardial injury of hypothyroidism.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第10期236-240,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
辽宁省教育厅项目(2009A492)
辽宁省教育厅优秀人才计划项目(LR201026)
辽宁省教育部博士点基金项目(20092133110004)
