摘要
目的:优选采用湿法超微粉碎法提取地龙蛋白的工艺。方法:在单因素试验基础上,以浸泡时间、料液比、提取温度、粉碎时间为影响因素,蛋白质得率为响应值,根据中心组合试验设计原理采用四因素三水平的响应面分析法,对湿法超微粉碎提取地龙蛋白条件进行优化。结果:湿法超微粉碎提取地龙蛋白的最佳工艺条件为浸泡时间4.7 h,料液比1︰11,提取温度17℃,粉碎时间10 min。在此条件下蛋白质得率3.46%。结论:湿法超微粉碎提取技术适用于地龙蛋白提取。该试验优选提取工艺稳定可行,适于工业化推广。
Objective： To optimize extraction technology of earthworm protein by wet ultrafine grinding technology. Method： On the basis of single-factor test, extraction technology of wet ultrafine grinding method on earthworm protein was optimized with soaking time, solid-liquid ratio, extraction temperature and grinding time as influencing factors, yield of protein as response value. According to central composite test design principles, 4 factors and 3 levels of response surface＇ analysis method was used. Result： Optimum extraction process of earthworm protein by wet uhrafine grinding technology was as follows： soaked 4.7 h, solid-liquid rate 1 ： 11, extraction temperature 17 ℃, grinding time 10 min. Under these conditions, yield of protein could be up to 3.46%. Conclusion： Wet uhrafine grinding technology was suitable for extraction of earthworm protein. This optimize extraction technology was stable, feasible and suitable for large-scale promotion.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第10期33-37,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
“重大新药创制”科技重大专项(2011ZX09401-308-008)
江苏省中医药局科技项目(LZ09007)
关键词
湿法超微粉碎
地龙
响应面分析法
蛋白质
提取工艺
wet ultrafine grinding
earthworm
response surface methodology
protein
extraction technology
