摘要
目的 探讨 ATP敏感性钾通道开放剂 (KCOs) Pinacidil (5 0μmol/ L )对 L angendorff灌注兔心模型超极化停跳的保护作用。方法 选用离体兔心脏 2 4个 ,随机分成 3组 :对照组 (C组 ) ;低温超极化组 (L H组 ) ;常温超极化组 (WH组 )。离体兔心 L angendorff模型灌注充氧的 Krebs- Henseleit (K- H)液稳定后 ,L H组或 WH组分别灌注含 Pinacidil的 4℃或 37℃的 St.Thomas(K+ 5 m mol/ L )停跳液 ,C组为不含 Pinacidil的 St.Thom as (K+ 16 m mol/ L )停跳液。全心脏缺血 40 m in,复灌 2 0 min。结果 L H组心脏停跳迅速 ,WH组停跳缓慢 ,但复跳迅速 ,与 C组比较 ,差异有显著性 (P<0 .0 1) ;再灌注后 L H组心率的恢复较慢 ,与 WH组一样 ,心肌收缩力与左心内压的恢复显著快于 C组 (P<0 .0 5 ) ;L H组与 WH组心肌组织ATP、总腺苷量 (TAN)、细胞能荷 (EC)显著高于 C组 ,而丙二醛 (MDA)的含量明显低于 C组 (P<0 .0 5或 0 .0 1)。结论 Pinacidil 5 0μm ol/ L诱导心脏超极化停跳 ,能够降低心肌 ATP的消耗 ,减少脂质过氧化物的形成 ,明显改善离体兔心缺血
Objective To evaluate the myocardial protective effect of hyperpolarized arrest induced with ATP-sensitive potassium channel opener ,pinacidil in vitroMethods Twenty-four rabbit hearts were randomly divided into three groups : hypothermic hyperpolarized group (LH group), normothermic hyperpolarized group ( WH group), and hyperkalemic control group (group C) The isolated rabbit hearts with a Langendorff apparatus were perfused oxygenated Krebs-Henseleit's solution (K-H) for 10 min and followed by cardioplegia The cardioplegia consisted of StThomas solution with either traditional high potassium (16mmol/L KCl, 4 ℃, group C), or pinacidil 50μmol/L (4 ℃ in LH group or 37 ℃ in WH group ) with 5mmol/L KCl The hearts were subjected to 40 min perfusional occlusion and followed by 20 min reperfusionResults Hearts were arrested quickly in LH group, and a little slowly in WH group, but rebeated quickly , which were different significantly from those in group C ; Recovery of LVP and left ventricle contracility in LH group and WH group were remarkedly faster than those in group C (P<005 ); Levels of ATP,TAN and EC were higher in LH and WH group than in group C (P<005 or 001 ); The MDA content was obviously lower in two hyperpolarized groups than in group C (P< 001 )Conclusions Hyperpolarized heart arrest induced with pinacidil can reduce ATP consumption and lipid peroxide formation of the myocardium ,and improve heart function recovery , providing myocardium protection superior to that of traditional depolarized hyperkalemic arrest
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2000年第6期365-368,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金资助项目!(NO.3 9760 0 71)
