摘要
目的了解血浆黏附分子颗粒膜蛋白（ＧＭＰ－１４０）水平及其在肾组织的表达与狼疮肾炎（ＬＮ）活动。肾脏病理及肾功能损害的关系。方法用酶联免疫吸附法（ＥＬＩＳＡ）测定血浆ＧＭＰ－１４０水平，并用免疫组织化学染色法，观察正常肾和狼疮肾组织ＧＭＰ－１４０表达。结果ＬＮ患者较正常人以及活动ＬＮ较非活动ＬＮ，血ＧＭＰ－１４０水平显著升高；ＬＮ紧组织ＧＭＰ－１４０表达较正常肾组织明显增强，且狼疮肾组织ＧＭＰ－１４０＋细胞数与血浆ＧＭＰ－１４０水平和狼疮肾活动指数明显正相关，与肾脏病理和功能损害有一定相关关系。结论ＧＭＰ－１４０在介导ＬＮ中起重要作用，其血浆水平或肾组织表达水平可作为反映ＬＮ活动和进行性肾损害的指标。
Objective To investigate the plasma level and in situ expression of granular membrane protein(GMP-140) in lupus nephritis (LN), and their correlations with the renal injuries. Methods Plasma GMP-140 was measured with ELISA in LN patients and normal control. GMP-140 expression in the kidney was measured by a microwave-based immunohistochemistry. Results There was a significant elevation of plasma GMP-140 level and marked up-regulation of GMP-140 expression in renal tissue of LN patients as compared to the normal control. The elevated level of plasma GMP-140 and its increased expression in were strongly correlated with LN activity, renal function and histological changes. Conclusion GMP-140 may play an important role in the mediation of LN pathogenesis and its plasma level and expression in renal tissue may be used as an index of the disease activity and progressive renal injuries.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2000年第3期169-172,共4页
Chinese Journal of Nephrology
基金
广东省中医药管理局基金!(98375)
关键词
狼疮肾炎
免疫组织化学
颗粒膜蛋白
Lupus nephritis
Immunohistochemistry
Granular membrane protein
