摘要
为研究黑杨的速生分子调控机制,采用PCR技术,从欧美杂种黑杨R270(Populus deltoides×Populus nigra)中克隆得到GASA基因,并构建其植物表达载体,通过农杆菌花序侵染法转入拟南芥,获得转基因植株,进行黑杨GASA基因的功能分析。结果表明:克隆得到的黑杨GASA基因cDNA片段总长为330 bp,可编码109个氨基酸残基;与野生型拟南芥相比,超量表达PdGASA4能促进拟南芥的提早抽薹、叶片的伸长及茎的增高和加粗。
In order to study the mechanisms of rapid growth in poplar,we cloned the GASA gene from Populus deltoides × Populus nigra,R270 by PCR.The paper also constructed its plant expression vector.Transgenic plants were obtained by the flower dipping method mediated by Agrobacterium tumefacien and analyzed the biological function of poplar GASA gene.The results showed that the PdGASA cDNA was 330 bp in length that was capable of encoding a protein of 109 amino acid;compared to the WT type,overexpression of PdGA20ox resulted in earlier flowering,larger leaf areas and increasing stem elongation.
出处
《广东农业科学》
CAS
CSCD
北大核心
2012年第8期138-140,144,F0002,共5页
Guangdong Agricultural Sciences
基金
国家重点基础研究发展计划项目(2009CB119101)
