摘要
目的：建立以高效液相色谱法测定经舒胶囊中丹皮酚含量的方法。方法：采用Platisil ODS色谱柱（4.6 mm×250 mm 5μm）;以甲醇-水（45：55）为流动相;柱温：40℃;流速：1.0ml.min-1;检测波长：274nm。结果：在建立的色谱条件下,丹皮酚进样量在0.08487~0.50922μg范围内与峰面积呈良好的线性关系（r=0.9999）,平均回收率（n=6）为98.75%（RSD=1.74%）。结论：方法简便可靠,分离度好,可用于经舒胶囊的质量控制。
Objective： To establish the HPLC method for the determination of paeonol in ＇JingShu＇ Capsule.Methods： The separation was performed on Platisil ODS（250 mm×4.6 mm,5 μm）column;The mobile phase was methanol-water（45：55） at a flow rate of 1.0ml.min-1;The detection wavelength was 274 nm,and the column temperature was 40℃.Results： The calibration curve showed good linearity in the range of 0.08487~0.50922 μg（r=0.9999）.The average recovery was 98.75%,RSD was 1.74%.Conclusion： The method is convenient,rapid and accurate.It could be used for control the quality of ＇JingShu＇ Capsule.
出处
《现代生物医学进展》
CAS
2012年第11期2163-2166,共4页
Progress in Modern Biomedicine
基金
project(81072398)supported by the National Natural Science Foundation of China~~
