摘要
目的探讨感染不同基因型HBV患者血清中自身抗体的存在状况及其分布特征，以了解HBV感染与自身免疫的关系。方法采用聚合酶链式反应限制性片段长度多态分析技术对重庆地区252例HBV感染者血清进行HBV基因分型，同时采用间接免疫荧光法或免疫斑点法检测其血清中的抗核抗体、抗线粒体抗体、抗平滑肌抗体、抗肝肾微粒体抗体、抗核糖核蛋白抗体、抗Sm、抗SS-A抗体、抗SS-B抗体、scl-70、抗Jo-1抗体等自身抗体。将患者分为慢性乙型肝炎组、肝硬化组和肝细胞癌组3组，并设立正常对照组，分别就自身抗体分布、性别及年龄等多种指标进行对比分析。计数资料采用疋。检验，3组间两两比较P值α校正为0．05／3=0．0176。结果252例患者血清中检出基因B型170例（67．460／0）、C型82例（32．540／0），未发现A、D、E、F、G、H基因型存在；其血清中检出多种自身抗体，总检出率为76．98％，与正常对照组（12．0％）比较，差异有统计学意义（χ^2=44．60，P〈0．05）;170例B基因型患者血清中131例（77．06％）检出自身抗体，82例C型患者血清中63例（76．83％）检出自身抗体，两组比较，差异无统计学意义（P〉0．05）。结论本地区HBV感染者基因型主要为B型和C型，以B型居多；乙型肝炎患者自身抗体阳性率较高，检出率与病程、年龄相关，而号陛别、病毒基因的类型等因素无关。
Objective To study the genotypes of hepatitis B virus （HBV） in patients from Chongqing district and determine the prevalence of autoantibodies in these patients. Methods HBV genotyping was carried out by restriction fragment length polymorphism analysis of venous blood serum samples from 252 chronic hepatitis B （CHB） patients and 25 healthy controls. Indirect immunofluorescent assay was used to detect autoantibodies, including aninuclear antibody, anti-mitochondrial antibody, anti-smooth muscle （SM） antibody, and anti-liver and kidney microsomal antibody. Immunospot assay was used to detect anti- ribonucleoproteirganti-SM antibodies, anti-SS-A antibody, anti-SS-B antibody, anti-sol-70 antibody, and anti- Jo-1 antibody. Correlations between the production of autoantibodies and patient age and sex and various genetypes of HBV were analyzed by the Chi-squared test. Results The most frequent HBV genotype in CI-IB patients was B （67.3%）, followed by genotype C （32.7%）. Genotypes A, D, E, F, G and H were not detected in any of the CHB patients. The positive rate of autoantibodies was higher in the CHB patients than in the healthy group （76.98% vs. 12.00%, χ2 = 44.60, P〈 0.05）. There was no significant differences in the autoantibodies profiles of CHB patients carrying the B or C genotypes （Z2=0.0016, P〉0.05）. Conclusion The main HBV genotypes in CHB patients in the Chongqing district are B and C. Autoantibodies are prevalent among theseCHB patients, and are correlated with patient age and course of liver disease but not HBV genotype or patient sex,
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2012年第6期448-452,共5页
Chinese Journal of Hepatology
基金
基金项目：国家自然科学基金（30972582）
重庆市自然科学基金（2009BB5276）
