摘要
为了解我国水貂肠炎病毒(MEV)的流行情况,本研究采用F81细胞从疑似患有肠炎的水貂粪便样品中分离出一株病毒,经形态学、血清学、动物回归试验和分子生物学鉴定,分离的病毒为MEV,命名为LN-10。对该病毒主要结构蛋白VP2基因进行克隆测序和基因进化分析表明,LN-10分离株VP2基因与GenBank中的其他18株MEV株核苷酸和氨基酸均有较高的同源性,分别为99.3%～100%和99%～100%,其中核苷酸同源性与ZYL-1株为100%,而氨基酸同源性与ZYL-1株和Manzhouli株均为100%。本研究为MEV分子流行病学调查和疫苗的研究奠定了基础。
To investigate the prevalence of mink enteritis virus （MEV）, we isolated a virus from mink with clinical signs of enteritis. This virus was identified as MEV by electron microscope examination, serologic test, artificial infection in minks and the VP2 gene sequencing （HQ694567）, designated LN-10. Furthermore, the phylogenetic analysis of the VP2 gene indicated that the MEV LN-10 VP2 gene shared higher homology with other 18 MEV strains available in GenBank. The homology of nucleotide and deduced amino acids were from 99.3% to 100% and 99% to 100%, respectively. The highest degree was 100% with ZYL-1 strain for nucleotide homology and 100% with ZYL-1 and Manzhouli strains for deduced amino acids homology. This study provided the basis evidence for molecular epidemiology investigation and development of vaccine against viral mink enteritis.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2012年第6期440-443,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
农业科技成果转化资金项目(2010GB2B100098)
吉林省重大科技成果转化项目(10ZDZH010)
关键词
水貂肠炎病毒
分离
鉴定
mink enteritis virus
isolation
identification
